![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody, Internal (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NBP2-16793-img0010.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody, Internal (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Histone Deacetylase 4/HDAC4. The exact sequence is proprietary. |
| Localization | Nucleus; Cytoplasm |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HDAC4 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | IHC-FR-Assay dependent |
|
| Theoretical MW | 119 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-16793 | Applications | Species |
|---|---|---|
| Sirchia SM, Faversani A, Rovina D et al. Epigenetic effects of chromatin remodeling agents on organotypic cultures Epigenomics 2016-03-01 [PMID: 26949823] (IHC-P, Human) | IHC-P | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|
-(01-ml)_NBP2-16793_9361.jpg)
Enlarge |
reviewed by:
Liyong Zhang |
WB | Human | 08/15/2014 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone Deacetylase 4/HDAC4 Antibody (NBP2-16793)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HDAC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/HDAC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16793-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HDAC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Human colon carcinoma, using HDAC4 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC4-Antibody-Immunohistochemistry-Paraffin-NBP2-16793-img0002.jpg "Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Human colon carcinoma, using HDAC4 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NBP2-16793-img0008.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NBP2-16793-img0009.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunoprecipitation: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of HDAC4 protein by 4 ug HDAC4 antibody [N3C1], Internal.](http://images.novusbio.com/fullsize/HDAC4-Antibody-Immunoprecipitation-NBP2-16793-img0006.jpg "Immunoprecipitation: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of HDAC4 protein by 4 ug HDAC4 antibody [N3C1], Internal.")
![Knockdown Validated: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Knockdown-Validated-NBP2-16793-img0011.jpg "Knockdown Validated: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]](https://images.novusbio.com/images2/RUNX2_MAB2006_Immunocytochemistry_13309.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-ml)_NBP2-16793_9361.jpg)