![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr-p84-Thoc1 Antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0011.jpg "Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr-p84-Thoc1 Antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, AvSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human hHpr1-p84-Thoc1. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | THOC1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ChIP assay- Assay dependent |
| Theoretical MW | 76 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for hHpr1-p84-Thoc1 Antibody (NBP2-16822)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16822-img0010.jpg "Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP2-16822-img0002.jpg "Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.")
![Chromatin Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-Nuclear Matrix Protein p84 antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Chromatin-Immunoprecipitation-NBP2-16822-img0007.jpg "Chromatin Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-Nuclear Matrix Protein p84 antibody.")
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0004.jpg "Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0009.jpg "Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 ug of Nuclear Matrix Protein p84 antibody [C1C3] 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunoprecipitation-NBP2-16822-img0006.jpg "Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 ug of Nuclear Matrix Protein p84 antibody [C1C3] 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
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