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Fes Antibody (678527)

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Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and HEL 92.1.7 human erythroleukemic cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Fes Monoclonal Antibody (Catalog # MAB6460) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fes at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and HEL 92.1.7 human erythroleukemic cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Fes Monoclonal Antibody ...read more
Fes was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Fes Monoclonal Antibody (Catalog # MAB6460) at 15 µg/mL overnight at 4 °C. Before incubation with the primary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
678527
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6460-SP
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Fes Antibody (678527) Summary

Immunogen
E. coli-derived recombinant human Fes
Ser270-Ala370
Accession # P07332
Specificity
Detects human Fes in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FES
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fes Antibody (678527)

  • EC 2.7.10
  • EC 2.7.10.2
  • feline sarcoma oncogene
  • feline sarcoma virus
  • Fes
  • FPS
  • Proto-oncogene c-Fes
  • Proto-oncogene c-Fps
  • proto-oncogene tyrosine-protein kinase Fes/Fps
  • tyrosine-protein kinase Fes/Fps

Background

Fes (homolog of feline sarcoma oncogene), also called c-FES or c-FPS, is a 92 kDa protein within subgroup IV of the non-receptor tyrosine kinase family. It is expressed in myeloid hematopoietic cells, vascular endothelium and neurons and regulates cell adhesion and extravasation. The 822 amino acid (aa) human Fes contains F-BAR (aa 7-239), SH2 (aa 460-549) and kinase (aa 561-822) domains. The F-BAR domain contains coiled-coil homo-oligomerization and microtubule binding sequences. Within the region used as an immunogen, human Fes shares 85% and 87% aa identity with mouse and rat Fes, respectively. Human isoforms of 764, 752, 694 and 681 aa may be missing aa 72-129 or 441-511 and/or contain an alternate 17 aa for aa 610-640, while an isoform of 891 aa contains 18 and 51 aa inserted after aa 510 and 568.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Fes Antibody
(678527)
MAB6460
Fes Antibody (678527)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FES
Uniprot