Home » Hepsin » Hepsin Antibodies » Hepsin Antibody (506230)
Novus Biologicals products are now on bio-techne.com

Hepsin Antibody (506230)

 Submit a Review

Images

 
(
Enlarge)
Hepsin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Hepsin Monoclonal Antibody (Catalog # MAB47761) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Hepsin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Hepsin Monoclonal Antibody (Catalog # MAB47761) at 25 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
506230
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB47761-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Hepsin Antibody (506230) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepsin
Ser46-Leu417
Accession # P05981
Specificity
Detects human Hepsin in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HPN
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hepsin Antibody (506230)

  • EC 3.4.21
  • EC 3.4.21.106
  • Hepsin
  • HPN
  • serine protease hepsin catalytic chain
  • serine protease hepsin
  • TMPRSS1
  • TMPRSS1serine protease hepsin non-catalytic chain
  • Transmembrane protease serine 1
  • transmembrane protease, serine 1

Background

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver but is also present in many other tissues; most notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII and may initiate blood coagulation at the cell surface (4). Hepsin is over-expressed in various human tumors including prostate (5) and is considered to be a biomarker for prostate cancer (6). Recombinant human (rh) Hepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the rhHepsin.

  1. Leytus, S.P. et al. (1988) Biochemistry 27:1067.
  2. Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
  3. Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
  4. Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
  5. Dhanasekaran, S.M. et al. (2001) Nature 412:822.
  6. Wu, Q. and G. Parry (2007) Front. Biosci. 12:5052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HAI-1/HGFA
Inhibitor 1 Antibody ...
AF1048
Western Blot HAI-1/HGFA Inhibitor 1 Antibody [Unconjugated]
Immunohistochemistry HAI-1/HGFA Inhibitor 1 Antibody [Unconjugated]
Simple Western HAI-1/HGFA Inhibitor 1 Antibody [Unconjugated]
(8 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
Kallikrein 3/PSA
[HRP]
DKK300
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
(17 Publications)
Species: Hu
Applications: ELISA
HVSL1 Antibody
NBP1-97768
Western Blot HVSL1 Antibody
Western Blot HVSL1 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
HGF [Unconjugated]
294-HG
Bioactivity HGF [Unconjugated]
Cell Culture HGF [Unconjugated]
(113 Publications)
Species: Hu
Applications: BA
HAI-2/SPINT2
Antibody [Unconjugate
AF1107
HAI-2/SPINT2 Antibody [Unconjugated]
(3 Publications)
Species: Mu
Applications: IHC, WB
TMPRSS2 Antibody
NBP1-20984
Western Blot TMPRSS2 Antibody
Immunohistochemistry-Paraffin TMPRSS2 Antibody
Immunohistochemistry-Paraffin TMPRSS2 Antibody
(8 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, Simple Western, WB
Prostasin/Prss8
Antibody [Unconjugate
AF4599
Western Blot Prostasin/Prss8 Antibody [Unconjugated]
Simple Western Prostasin/Prss8 Antibody [Unconjugated]
Immunohistochemistry Prostasin/Prss8 Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: IHC, IP, Simple Western, WB

alpha-Methylacyl-CoA R ...
NBP1-87168
Immunohistochemistry-Paraffin alpha-Methylacyl-CoA Racemase/AMACR Antibody
Immunohistochemistry-Paraffin alpha-Methylacyl-CoA Racemase/AMACR Antibody
Immunohistochemistry-Paraffin alpha-Methylacyl-CoA Racemase/AMACR Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Corin [Biotin]
DY2209
N/A Corin [Biotin]
(8 Publications)
Species: Hu
Applications: ELISA
Protein S/PROS1
Antibody [Unconjugate
AF4036
Western Blot Protein S/PROS1 Antibody [Unconjugated]
Simple Western Protein S/PROS1 Antibody [Unconjugated]
(7 Publications)
Species: Hu
Applications: Simple Western, WB
PLAG1 Antibody
(3B7)
H00005324-M02
Immunohistochemistry PLAG1 Antibody (3B7)
Immunohistochemistry PLAG1 Antibody (3B7)
ELISA PLAG1 Antibody (3B7)
(20 Publications)
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, WB
u-Plasminogen
Activator/Urokinase [ ...
1310-SE
Enzyme Activity u-Plasminogen Activator/Urokinase [Unconjugated]
(14 Publications)
Species: Hu
Applications: EnzAct
PSAT1 Antibody
NBP1-32920
Western Blot PSAT1 Antibody
Immunocytochemistry/ Immunofluorescence PSAT1 Antibody
Immunohistochemistry-Paraffin PSAT1 Antibody
(12 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB

Puromycin-sensitive am ...
NBP2-13669
Immunohistochemistry-Paraffin Puromycin-sensitive aminopeptidase/NPEPPS Antibody
Immunohistochemistry Puromycin-sensitive aminopeptidase/NPEPPS Antibody
Immunohistochemistry-Paraffin Puromycin-sensitive aminopeptidase/NPEPPS Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(664 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
Matriptase/ST14
Antibody [Unconjugate
AF3946
Intracellular Staining by Flow Cytometry Matriptase/ST14 Antibody [Unconjugated]
Simple Western Matriptase/ST14 Antibody [Unconjugated]
Simple Western Matriptase/ST14 Antibody [Unconjugated]
(19 Publications)
Species: Hu
Applications: CyTOF-ready, ICFlow, WB

Publications for Hepsin Antibody (MAB47761) (0)

There are no publications for Hepsin Antibody (MAB47761).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Hepsin Antibody (MAB47761) (0)

There are no reviews for Hepsin Antibody (MAB47761). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Hepsin Antibody (MAB47761) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Hepsin Products

Blogs on Hepsin

There are no specific blogs for Hepsin, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Hepsin Antibody (506230) and receive a gift card or discount.


Bioinformatics

Gene Symbol HPN
Uniprot