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Prostasin/Prss8 Antibody [Unconjugated]

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Western blot shows lysates of human prostate tissue, LNCaP human prostate cancer cell line, PC-3 human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostasin/Prss8 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human prostate tissue, LNCaP human prostate cancer cell line, PC-3 human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of ...read more
Prostasin/Prss8 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 10 µg/mL Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of human prostate tissue and LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Prostasin/Prss8 at approximately 50 kDa (as ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4599
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4599-SP
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Prostasin/Prss8 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Prostasin
Ala33-Gly319
Accession # Q16651
Specificity
Detects human Prostasin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse Prostasin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PRSS8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using AF4599.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prostasin/Prss8 Antibody [Unconjugated]

  • CAP1
  • channel-activating protease 1
  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.120
  • Prostasin
  • protease, serine, 8
  • Prss8
  • Serine protease 8

Background

Prostasin, also known as channel activating protease 1, is a serine protease with trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of Prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of Prostasin is involved in the regulation of epithelial sodium channels (4, 5).

  1. Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.
  2. Yu, J.X. et al. (1994) J. Biol. Chem. 269:18843.
  3. Yu, J.X. et al. (1995) J. Biol. Chem. 270:13483.
  4. Caldwell, R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C194.
  5. Tong, Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Prostasin/Prss8
Antibody [Unconjugate
AF4599
Prostasin/Prss8 Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC, IP

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Bioinformatics

Gene Symbol PRSS8
Uniprot