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Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free

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Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ELISA, IHC, IP, AP
Clone
A74
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free Summary

Immunogen
Bovine corneal endothelial cells
Epitope
Epitope is located in domain V
Specificity
Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPG2
Purity
Protein A or G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunoaffinity Purification
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
ELISA: NBP2-23523 can be used in ELISA. WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1) IHC: NBP2-23523 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. AP: NBP2-23523 can be used in affinity chromatography, in conjunction with NBP2-23522 to separate recombinant domain I from full length perlecan. (1) IP: NBP2-23523 can be used in immunoprecipitation.
Publications
Read Publications using
NBP2-23523 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free

  • basement membrane-specific heparan sulfate proteoglycan core protein
  • endorepellin (domain V region)
  • heparan sulfate proteoglycan 2
  • HSPG
  • perlecan proteoglycan
  • perlecan
  • PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia)
  • PRCAN
  • SJA
  • SJS
  • SJS1

Background

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Heparan Sulfate Proteoglycan 2 Antibody (NBP2-23523)(3)

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Bioinformatics

Gene Symbol HSPG2