Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPG2
Purity
Protein A or G purified
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ELISA: NBP2-23523 can be used in ELISA. WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1) IHC: NBP2-23523 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. AP: NBP2-23523 can be used in affinity chromatography, in conjunction with NBP2-23522 to separate recombinant domain I from full length perlecan. (1) IP: NBP2-23523 can be used in immunoprecipitation.
Publications
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Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling .
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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