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PLC-gamma 1 Antibody (3H1C10) - BSA Free

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Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mouse mAb against NIH/3T3 (1), Jurkat (2), A431 (3), and Hela (4) cell lysate.
Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mouse mAb against NIH/3T3 (1), Jurkat (2), A431 (3), and Hela (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of Hela cells using PLCG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with ...read more
Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of paraffin-embedded esophagus tissues using PLCG1 mouse mAb with DAB staining.
Flow Cytometry: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of Raji cells using PLCG1 mouse mAb (green) and negative control (red).
Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 1192-1291)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mAb against human PLCG1 (AA: 1192-1291) recombinant protein. (Expected MW is 37.5 kDa)
Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of paraffin-embedded breast cancer tissues using PLCG1 mouse mAb with DAB staining.
ELISA: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
3H1C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52533-0.025mg
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PLC-gamma 1 Antibody (3H1C10) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PLC-gamma 1 (AA: 1192-1291) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLCG1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
148.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PLC-gamma 1 Antibody (3H1C10) - BSA Free

  • EC 3.1.4.11
  • gamma 1 (formerly subtype 148)
  • monophosphatidylinositol phosphodiesterase
  • NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
  • phosphatidylinositol phospholipase C
  • phosphoinositidase C
  • phosphoinositide phospholipase C
  • Phosphoinositide phospholipase C-gamma-1
  • phospholipase C, gamma 1
  • phospholipase C-148
  • Phospholipase C-gamma-1
  • Phospholipase C-II
  • PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
  • PLC148
  • PLC-148
  • PLCG1
  • PLCgamma 1
  • PLC-gamma 1
  • PLC-gamma-1
  • PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
  • PPLCA
  • triphosphoinositide phosphodiesterase

Background

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups (1). Phospholipase C gamma 1 (PLC-gamma 1) hydrolyses phosphatidylinositol-4,5-bisphosphate to the second messengers inositol-1,4,5-trisphosphate and diacylglycerol. PLC-gamma 1 also has mitogenic activity upon growth-factor-dependent tyrosine phosphorylation; however, this activity is not dependent on the phospholipase activity of PLC-gamma 1, but requires an SH3 domain (2). Also, results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PLCG1