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Heparan Sulfate Proteoglycan 2 Antibody (A74) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ELISA, IHC, IP, AP
Clone
A74
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-23523AF350
Alexa Fluor 405
NBP2-23523AF405
Alexa Fluor 488
NBP2-23523AF488
Alexa Fluor 532
NBP2-23523AF532
Alexa Fluor 594
NBP2-23523AF594
Alexa Fluor 647
NBP2-23523AF647
Alexa Fluor 700
NBP2-23523AF700
Alexa Fluor 750
NBP2-23523AF750
Biotin
NBP2-23523B
CoraFluor 1
NBP2-23523CL1
DyLight 350
NBP2-23523UV
DyLight 405
NBP2-23523V
DyLight 488
NBP2-23523G
DyLight 550
NBP2-23523R
DyLight 594
NBP2-23523DL594
DyLight 650
NBP2-23523C
DyLight 680
NBP2-23523FR
DyLight 755
NBP2-23523IR
FITC
NBP2-23523F
HRP
NBP2-23523H
Janelia Fluor 525
NBP2-23523JF525
Janelia Fluor 549
NBP2-23523JF549
Janelia Fluor 585
NBP2-23523JF585
Janelia Fluor 635
NBP2-23523JF635
Janelia Fluor 646
NBP2-23523JF646
PerCP
NBP2-23523PCP
Unconjugated
NBP2-23523-0.1ml
mFluor Violet 450 SE
NBP2-23523MFV450
mFluor Violet 500 SE
NBP2-23523MFV500
mFluor Violet 610 SE
NBP2-23523MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-23523-0.1ml
BSA Free
NBP2-23523-0.1ml
BSA Free
NBP2-23523-0.025ml
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Heparan Sulfate Proteoglycan 2 Antibody (A74) [Janelia Fluor® 669] Summary

Immunogen
Bovine corneal endothelial cells
Epitope
Epitope is located in domain V
Specificity
Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPG2
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Heparan Sulfate Proteoglycan 2 Antibody (A74) [Janelia Fluor® 669]

  • basement membrane-specific heparan sulfate proteoglycan core protein
  • endorepellin (domain V region)
  • heparan sulfate proteoglycan 2
  • HSPG
  • perlecan proteoglycan
  • perlecan
  • PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia)
  • PRCAN
  • SJA
  • SJS
  • SJS1

Background

Heparan sulfate proteoglycan is a major component of basement membranes, where the molecule may be involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. This form of HSPG, known as HSPG2 or perlecan, is encoded by a gene that maps to chromosome 1. The gene for the form of HSPG associated with the cell surface of fibroblasts has been mapped to human chromosome 8 (MIM 142460).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Heparan Sulfate
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NBP2-23523JF669
Heparan Sulfate Proteoglycan 2 Antibody (A74) [Janelia Fluor® 669]
Species: Hu, Bv
Applications: WB, ELISA, IHC, IP, AP

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Bioinformatics

Gene Symbol HSPG2