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Guanylate kinase Antibody

Images

 
Western Blot: Guanylate kinase Antibody [NBP2-14078] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: Guanylate kinase Antibody [NBP2-14078] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Guanylate kinase Antibody [NBP2-14078] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Guanylate kinase Antibody [NBP2-14078] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Guanylate kinase Antibody [NBP2-14078] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Guanylate kinase Antibody [NBP2-14078] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.
Independent Antibodies: Analysis using NBP2-14078 (A) shows similar pattern to independent antibody NBP2-76521 (B).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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Guanylate kinase Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: LRPIYISVQPPSLHVLEQRLRQRNTETEESLVKRLAAAQADMESSKEPGLFDVVIINDSLDQAYAELKEALSEEIKKAQRTGA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GUK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Guanylate kinase Protein (NBP2-14078PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Guanylate kinase Antibody

  • ATP:GMP Phosphotransferase
  • Deoxyguanylate Kinase
  • EC 2.7.4.8
  • FLJ42686
  • FLJ43710
  • GMK
  • GMP Kinase
  • Guanosine monophosphate Kinase
  • guanylate kinase 1
  • Guanylate Kinase
  • GUK1

Background

Essential for recycling GMP and indirectly, cGMP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GUK1