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GTF2H1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Analysis in human testis and skeletal muscle tissues using NBP2-38556 antibody. Corresponding GTF2H1 RNA-seq data are presented ...read more
Immunocytochemistry/ Immunofluorescence: GTF2H1 Antibody [NBP2-38556] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human skeletal muscle shows no nuclear positivity in myocytes.
Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.
Western Blot: GTF2H1 Antibody [NBP2-38556] - Analysis in human cell line U-2197.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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GTF2H1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYN
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GTF2H1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GTF2H1 Protein (NBP2-38556PEP)
Publications
Read Publication using NBP2-38556.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GTF2H1 Antibody

  • Basic transcription factor 2 62 kDa subunit
  • BTF2 p62
  • BTF2general transcription factor IIH, polypeptide 1 (62kD subunit)
  • General transcription factor IIH polypeptide 1
  • general transcription factor IIH subunit 1
  • general transcription factor IIH, polypeptide 1, 62kDa
  • TFB1
  • TFIIH basal transcription factor complex p62 subunit
  • TFIIH

Background

GTF2H1 is a component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNAand, when complexed to CAK, in RNA transcription by RNA polymerase II

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GTF2H1 Antibody (NBP2-38556)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GTF2H1 Antibody (NBP2-38556) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GTF2H1
Uniprot