Novus Biologicals products are now on bio-techne.com

DOK1 Antibody - BSA Free

Images

 
Western Blot: DOK1 Antibody [NBP1-77064] - Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: DOK1 Antibody [NBP1-77064] - Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: DOK1 Antibody [NBP1-77064] - K562 cells with DOK1 antibody at 2 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price

DOK1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1. The immunogen is located within amino acids 410 - 460 of DOK1. Amino Acid Squence: PQGPAFPEPGTATGS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DOK1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Western Blot 1 ug/ml
Application Notes
A 62 kDa band should be detected in Western Blot.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DOK1 Peptide (NBP1-77064PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for DOK1 Antibody - BSA Free

  • docking protein 1 (downstream of tyrosine kinase 1)
  • docking protein 1
  • docking protein 1, 62kD (downstream of tyrosine kinase 1)
  • docking protein 1, 62kDa (downstream of tyrosine kinase 1)
  • Downstream of tyrosine kinase 1
  • MGC117395
  • MGC138860
  • p62(dok)
  • P62DOK
  • pp62

Background

Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19151
Species: Bv, Hu, Ma
Applications: ICC/IF, IHC, IHC-P, IP, WB
H00008878-M01
Species: Bv, Ha, Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-31381
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-37399
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
H00002965-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, S-ELISA, WB
NBP2-02627
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AF5094
Species: Hu, Mu, Rt
Applications: WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NBP2-71407
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, WB
AF5414
Species: Hu
Applications: Simple Western, WB
NBP2-37490
Species: Hu, Pm, Pm
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
NBP3-16226
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-02434
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-25200
Species: Hu
Applications: B/N, Flow, IHC, IHC-Fr, WB
NBP1-77064
Species: Hu
Applications: WB, ELISA, ICC/IF

Publications for DOK1 Antibody (NBP1-77064) (0)

There are no publications for DOK1 Antibody (NBP1-77064).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DOK1 Antibody (NBP1-77064) (0)

There are no reviews for DOK1 Antibody (NBP1-77064). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DOK1 Antibody (NBP1-77064) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional DOK1 Products

Research Areas for DOK1 Antibody (NBP1-77064)

Find related products by research area.

Blogs on DOK1

There are no specific blogs for DOK1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DOK1 Antibody - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol DOK1
Uniprot