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RAP30 Antibody

Images

 
Western Blot: RAP30 Antibody [NBP3-17973] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-459
Immunocytochemistry/ Immunofluorescence: RAP30 Antibody [NBP3-17973] - Staining of human cell line U-2 OS shows positivity in nucleus and cytoskeleton (microtubules).
Immunohistochemistry: RAP30 Antibody [NBP3-17973] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Immunocytochemistry/ Immunofluorescence: RAP30 Antibody [NBP3-17973] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & microtubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RAP30 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GTF2F2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RAP30 Antibody

  • ATP-dependent helicase GTF2F2
  • BTF4
  • General transcription factor IIF 30 kDa subunit
  • general transcription factor IIF subunit 2
  • general transcription factor IIF, polypeptide 2, 30kDa
  • TF2F2
  • TFIIF
  • TFIIF-beta
  • Transcription initiation factor IIF subunit beta
  • Transcription initiation factor RAP30

Background

Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription preinitiation complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GTF2F2