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GSK-3 alpha/beta Antibody [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT-29 human colon adenocarcinoma cell line, MDA-MB-468 and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/ Rat GSK-3a/ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for GSK-3a/ beta at approximately 46 and 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT-29 human colon adenocarcinoma cell line, MDA-MB-468 and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL ...read more
GSK-3a/ beta was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat GSK-3a/ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2157) at 10 ...read more
HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human/Mouse/Rat GSK‑3 alpha / beta Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2157, filled histogram) or control ...read more
GSK3 binds to scaffold formed by histidine-rich cytoplasmic loop domains within the ZIP6-ZIP10 heteromer.(a) Domain organization of ZIP10 and ZIP6 and deletion constructs used in this work to test the hypothesis that a ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2157-SP
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GSK-3 alpha/beta Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GSK-3 beta
Accession # P49841
Specificity
Detects human, mouse, and rat GSK-3 beta and the closely related GSK-3 alpha .
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSK3A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2157 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSK-3 alpha/beta Antibody [Unconjugated]

  • DKFZp686D0638
  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 alpha
  • glycogen synthase kinase-3 alpha
  • GSK-3 alpha
  • GSK-3 alpha/beta

Background

GSK-3 is a Ser/Thr kinase first identified as an inactivator of Glycogen Synthase. GSK-3 acts as a multifunctional downstream switch that determines the output of numerous signaling pathways. There are two mammalian GSK-3 isoforms encoded by distinct genes, GSK-3 alpha and GSK-3 beta, which are structurally similar, but functionally non-identical. GSK-3a is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3 alpha and GSK-3 beta share 85% amino acid identity. Dysregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer's disease, bipolar disorder, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF2157
GSK-3 alpha/beta Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: WB, CyTOF-ready, Flow, ICC/IF

Publications for GSK-3 alpha/beta Antibody (AF2157)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Flow Cytometry, IHC, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC
(1)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
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Human
(2)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF2157 Applications Species
Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse) IHC Mouse
S Li, DM Pritchard, LG Yu Galectin-3 promotes secretion of proteases that decrease epithelium integrity in human colon cancer cells Cell Death & Disease, 2023-04-13;14(4):268. 2023-04-13 [PMID: 37055381] (Western Blot, Human) Western Blot Human
D Brethour, M Mehrabian, D Williams, X Wang, F Ghodrati, S Ehsani, EA Rubie, JR Woodgett, J Sevalle, Z Xi, E Rogaeva, G Schmitt-Ul A ZIP6-ZIP10 heteromer controls NCAM1 phosphorylation and integration into focal adhesion complexes during epithelial-to-mesenchymal transition Sci Rep, 2017-01-18;7(0):40313. 2017-01-18 [PMID: 28098160] (Immunoprecipitation, Western Blot, Mouse) Immunoprecipitation, Western Blot Mouse
Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response. PLoS ONE, 2014-01-28;9(1):e86466. 2014-01-28 [PMID: 24489729] (Western Blot, Human) Western Blot Human
Lapid K, Itkin T, D'Uva G, Ovadya Y, Ludin A, Caglio G, Kalinkovich A, Golan K, Porat Z, Zollo M, Lapidot T GSK3beta regulates physiological migration of stem/progenitor cells via cytoskeletal rearrangement. J Clin Invest, 2013-03-08;123(4):1705-17. 2013-03-08 [PMID: 23478410] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation. J. Exp. Med., 2010-03-15;207(4):867-80. 2010-03-15 [PMID: 20231377] (Western Blot, Rat) Western Blot Rat

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Bioinformatics

Gene Symbol GSK3A
Uniprot