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GSK-3 alpha Antibody (288901) [Unconjugated]

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GSK-3a was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human GSK-3a Monoclonal Antibody (Catalog # MAB2986) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GSK-3a was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human GSK-3a Monoclonal Antibody (Catalog # MAB2986) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
288901
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2986-SP
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GSK-3 alpha Antibody (288901) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GSK-3 alpha
Gly90-Ser483
Accession # P49840.2
Specificity
Detects human GSK-3 alpha . In direct ELISAs, this antibody does not detect recombinant human GSK-3 beta .
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GSK3A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSK-3 alpha Antibody (288901) [Unconjugated]

  • DKFZp686D0638
  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 alpha
  • glycogen synthase kinase-3 alpha
  • GSK3 alpha
  • GSK-3 alpha
  • GSK3A

Background

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 alpha is inhibited by phosphorylation at S21 by Akt and other kinases. Deregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer’s disease, bipolar disorder, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GSK-3 alpha Antibody (288901) [Unconjugated]
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Bioinformatics

Gene Symbol GSK3A
Uniprot