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GRP78/HSPA5 Antibody [DyLight 650]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, SyHa, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-06277AF350
Alexa Fluor 405
NBP1-06277AF405
Alexa Fluor 488
NBP1-06277AF488
Alexa Fluor 532
NBP1-06277AF532
Alexa Fluor 594
NBP1-06277AF594
Alexa Fluor 647
NBP1-06277AF647
Alexa Fluor 700
NBP1-06277AF700
Alexa Fluor 750
NBP1-06277AF750
Allophycocyanin
NBP1-06277APC
Biotin
NBP1-06277B
DyLight 350
NBP1-06277UV
DyLight 405
NBP1-06277V
DyLight 488
NBP1-06277G
DyLight 550
NBP1-06277R
DyLight 594
NBP1-06277DL594
DyLight 680
NBP1-06277FR
DyLight 755
NBP1-06277IR
FITC
NBP1-06277F
HRP
NBP1-06277H
Janelia Fluor 549
NBP1-06277JF549
Janelia Fluor 646
NBP1-06277JF646
PE
NBP1-06277PE
PerCP
NBP1-06277PCP
Unconjugated
NBP1-06277
mFluor Violet 450 SE
NBP1-06277MFV450
mFluor Violet 500 SE
NBP1-06277MFV500
mFluor Violet 610 SE
NBP1-06277MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-06277
BSA Free
NBP1-06277SS
BSA Free
NBP1-06277
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GRP78/HSPA5 Antibody [DyLight 650] Summary

Immunogen
Synthetic peptide made to a C-terminal portion of rat GRP78 (within residues 600-654). [Swiss-Prot# P06761]
Localization
Endoplasmic reticulum lumen. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Marker
ER Stress Marker
Predicted Species
Primate (92%), Golden Syrian Hamster (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for GRP78/HSPA5 Antibody [DyLight 650]

  • 78 kDa glucose-regulated protein
  • BIP
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • FLJ26106
  • GRP78
  • GRP-78
  • GRP78BIP
  • Heat shock 70 kDa protein 5
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • HSP70-5
  • HSPA5
  • Immunoglobulin heavy chain-binding protein
  • MIF2

Background

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell. BiP is also overexpressed in many cancers and has been hypothesized to actively regulate multiple malignant phenotypes including cell growth, migration, and invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GRP78/HSPA5
Antibody [DyLight 650
NBP1-06277C
GRP78/HSPA5 Antibody [DyLight 650]
Species: Hu, Mu, Rt, SyHa, Pm
Applications: WB, Simple Western, ICC/IF

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GRP78 - molecular chaperone and negative regulator of the unfolded protein response
The 78 kDa glucose-regulated protein (GRP78) is the eukaryotic orthologue to the prokaryotic heat shock 70 kDa protein 5 (HSPA5). GRP78 is also sometimes referred to as BiP. GRP78 is a member of the HSP70 family and plays dynamic roles in protein ...  Read full blog post.
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Bioinformatics

Gene Symbol HSPA5