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GRP78/HSPA5 Antibody [DyLight 550]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, Ce, Ch, Dr, Sh, Ze, Bv, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-06274AF350
Alexa Fluor 405
NBP1-06274AF405
Alexa Fluor 488
NBP1-06274AF488
Alexa Fluor 532
NBP1-06274AF532
Alexa Fluor 594
NBP1-06274AF594
Alexa Fluor 647
NBP1-06274AF647
Alexa Fluor 700
NBP1-06274AF700
Alexa Fluor 750
NBP1-06274AF750
Allophycocyanin
NBP1-06274APC
Biotin
NBP1-06274B
CoraFluor 1
NBP1-06274CL1
DyLight 350
NBP1-06274UV
DyLight 405
NBP1-06274V
DyLight 488
NBP1-06274G
DyLight 594
NBP1-06274DL594
DyLight 650
NBP1-06274C
DyLight 680
NBP1-06274FR
DyLight 755
NBP1-06274IR
FITC
NBP1-06274F
HRP
NBP1-06274H
Janelia Fluor 525
NBP1-06274JF525
Janelia Fluor 549
NBP1-06274JF549
Janelia Fluor 585
NBP1-06274JF585
Janelia Fluor 635
NBP1-06274JF635
Janelia Fluor 646
NBP1-06274JF646
Janelia Fluor 669
NBP1-06274JF669
PE
NBP1-06274PE
PerCP
NBP1-06274PCP
Unconjugated
NBP1-06274
mFluor Violet 450 SE
NBP1-06274MFV450
mFluor Violet 500 SE
NBP1-06274MFV500
mFluor Violet 610 SE
NBP1-06274MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-06274
BSA Free
NBP1-06274SS
BSA Free
NBP1-06274
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GRP78/HSPA5 Antibody [DyLight 550] Summary

Immunogen
Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]
Localization
Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Marker
ER Stress Marker
Predicted Species
Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Zebrafish reported in scientific literature (PMID:34327238) C. elegans reactivity reported in scientific literature (PMID: 31398187).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for GRP78/HSPA5 Antibody [DyLight 550]

  • 78 kDa glucose-regulated protein
  • BIP
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • FLJ26106
  • GRP78
  • GRP-78
  • GRP78BIP
  • Heat shock 70 kDa protein 5
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • HSP70-5
  • HSPA5
  • Immunoglobulin heavy chain-binding protein
  • MIF2

Background

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell. BiP is also overexpressed in many cancers and has been hypothesized to actively regulate multiple malignant phenotypes including cell growth, migration, and invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GRP78/HSPA5
Antibody [DyLight 550
NBP1-06274R
GRP78/HSPA5 Antibody [DyLight 550]
Species: Hu, Mu, Rt, Ce, Ch, Dr, Sh, Ze, Bv, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC

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GRP78 - molecular chaperone and negative regulator of the unfolded protein response
The 78 kDa glucose-regulated protein (GRP78) is the eukaryotic orthologue to the prokaryotic heat shock 70 kDa protein 5 (HSPA5). GRP78 is also sometimes referred to as BiP. GRP78 is a member of the HSP70 family and plays dynamic roles in protein ...  Read full blog post.
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Bioinformatics

Gene Symbol HSPA5