GRP78/HSPA5 Antibody - BSA Free

Within the ER misfolded proteins are detected by Bip(GPR78). In the presence of misfolded proteins, Bip disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized, phosphorylated form PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. Bip also is binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulated transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated, misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-P, WB, Western Blot.

Showing Publications 1 - 10 of 10.

Publications using NB300-520	Applications	Species
Veneri FA, Prada V, Mastrangelo R et al. A novel mouse model of CMT1B identifies hyperglycosylation as a new pathogenetic mechanism Human molecular genetics 2022-07-31 [PMID: 35908287] (WB, Mouse)	WB	Mouse
Bai Y, Treins C, Volpi VG et al. Treatment with IFB-088 Improves Neuropathy in CMT1A and CMT1B Mice Molecular Neurobiology 2022-07-01 [PMID: 35501630] (Western Blot)	Western Blot
Della-Flora Nunes G, Wilson ER, Hurley E et al. Activation of mTORC1 and c-Jun by Prohibitin1 loss in Schwann cells may link mitochondrial dysfunction to demyelination eLife 2021-09-14 [PMID: 34519641] (WB, Rat)	WB	Rat
Della-Flora Nunes G, Wilson ER, Marziali LN et al. Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells Nature communications 2021-06-02 [PMID: 34078899] (WB, Mouse)	WB	Mouse
Scapin C, Ferri C, Pettinato E et al. Phosphorylation of eIF2 alpha Promotes Schwann Cell Differentiation and Myelination in CMT1B Mice with Activated UPR J Neurosci 2020-10-14 [PMID: 32973043] (Mouse)		Mouse
Wang G, Xu J, Zhao J et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice Nat Commun 2020-01-10 [PMID: 31924786] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Chapados NA, Boucher MP. Liver metabolic disruption induced after a single exposure to PCB126 in rats. Environ Sci Pollut Res Int. 2016-10-31 [PMID: 27796995] (WB, Rat)	WB	Rat
Magarin M, Pohl T, Lill A et al. Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress J Mol Cell Cardiol 2016-04-24 [PMID: 27106802] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Musner N, Sidoli M, Zambroni D et al. Perk Ablation Ameliorates Myelination in S63del-Charcot-Marie-Tooth 1B Neuropathy. ASN Neuro. 2016-04-01 [PMID: 27095827] (WB, Mouse)	WB	Mouse
Shin D, Feltri ML, Wrabetz L. Altered Trafficking and Processing of GALC Mutants Correlates with Globoid Cell Leukodystrophy Severity. J. Neurosci. 2016-02-10 [PMID: 26865610] (WB, Human)	WB	Human

Array NB300-520

• GRP78/HSPA5 Antibodies
• GRP78/HSPA5 ELISA Kits
• GRP78/HSPA5 Lysate
• GRP78/HSPA5 Proteins