![Knockout Validated: GOLM1 Antibody [NBP1-50627] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and GOLM1 knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human GOLM1 polyclonal Antibody (Catalog # NBP1-50627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for GOLM1 at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Knockout-Validated-NBP1-50627-img0009.jpg "Knockout Validated: GOLM1 Antibody [NBP1-50627] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and GOLM1 knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human GOLM1 polyclonal Antibody (Catalog # NBP1-50627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for GOLM1 at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.61 mg/ml |
| Immunogen | A genomic peptide made to an internal region of the human GOLM1 protein (within residues 250-400). [Swiss-Prot Q8NBJ4] |
| Localization | Golgi apparatus - cis-Golgi network membrane |
| Marker | Golgi Apparatus Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GOLM1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.1% BSA, 50% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.61 mg/ml |
| Purity | Immunogen affinity purified |
![Simple Western alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/16253.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_21503.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_22402.jpg)
| Publications using NBP1-50627 | Applications | Species |
|---|---|---|
| Muralidharan C, Huang F, Enriquez J et al. Suppression of the Integrated Stress Response in Islet ? Cells Decreases Risk of Autoimmune Diabetes bioRxiv 2023-10-09 (WB) | WB | |
| Nagaraj M, HOring M, Ahonen MA et al. GOLM1 depletion modifies cellular sphingolipid metabolism and adversely affects cell growth Journal of lipid research 2022-08-07 [PMID: 35948172] (ICC/IF, WB, KD, Human) | ICC/IF, WB, KD | Human |
| Garcia V, Park E. J, et al. Unbiased proteomics identifies plasminogen activator inhibitor-1 as a negative regulator of endothelial nitric oxide synthase. Proc Natl Acad Sci U S A 2020-04-28 [PMID: 32300005] (ICC/IF, Human) | ICC/IF | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Human | 12/15/2017 |
Summary
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-(01-ml)_NBP1-50627_8246.bmp)
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reviewed by:
Bryan Tinsley |
ICC | Human | 06/12/2014 |
Summary
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-(01-ml)_NBP1-50627_8241.jpg)
Enlarge |
reviewed by:
Bryan Tinsley |
WB | Human | 06/12/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for GOLM1 Antibody (NBP1-50627)Find related products by research area.
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| Verified Customer 12/15/2017 |
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| Application: | WB | |
| Species: | Human |
| Bryan Tinsley 06/12/2014 |
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| Application: | ICC | |
| Species: | Human |
| Bryan Tinsley 06/12/2014 |
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| Application: | WB | |
| Species: | Human |
![Western Blot: GOLM1 Antibody [NBP1-50627] - Western Blot Image of GOLM1. Whole cell protein from HeLa (lane 1), 3T3 (lane 2), MEF (lane 3), Raw264.7 (lane 4), L929 (lane 5), Neuro2A (lane 6) and PC12 (lane 7) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-GOLM1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Western-Blot-NBP1-50627-img0008.jpg "Western Blot: GOLM1 Antibody [NBP1-50627] - Western Blot Image of GOLM1. Whole cell protein from HeLa (lane 1), 3T3 (lane 2), MEF (lane 3), Raw264.7 (lane 4), L929 (lane 5), Neuro2A (lane 6) and PC12 (lane 7) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-GOLM1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - Confocal immunofluorescent analysis of HeLa cells using GOLM1 antibody (NBP1-50627, 1:25). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50627-img0007.jpg "Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - Confocal immunofluorescent analysis of HeLa cells using GOLM1 antibody (NBP1-50627, 1:25). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).")
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - ICC staining of GOLM1 in HEK293T cells with FITC (green). Nuclei (blue) were counterstained with Dapi.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50627-img0004.jpg "Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - ICC staining of GOLM1 in HEK293T cells with FITC (green). Nuclei (blue) were counterstained with Dapi.")
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - GOLM1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50627-img0003.jpg "Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-50627] - GOLM1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
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![Western Blot Protein S/PROS1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Protein_S_AF4036_Western_Blot_17082.jpg)
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![Immunohistochemistry Clusterin/APOJ Antibody [Unconjugated]](https://images.novusbio.com/images2/Clusterin_AF2747_Immunohistochemistry_9945.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-ml)_NBP1-50627_8246.bmp)
-(01-ml)_NBP1-50627_8241.jpg)