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GM130/GOLGA2 Antibody

Images

 
Independent Antibodies: Western Blot: GM130/GOLGA2 Antibody [NBP1-89757] - Analysis using Anti-GOLGA2 antibody NBP1-89757 (A) shows similar pattern to independent antibody NBP1-89756 (B).
Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody [NBP1-89757] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry: GM130/GOLGA2 Antibody [NBP1-89757] - Immunohistochemical staining of human lymph node shows low expression as expected.
Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human parathyroid gland shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining in human parathyroid gland and lymph node tissues using anti-GOLGA2 antibody. Corresponding GOLGA2 RNA-seq data ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-GOLGA2 antibody NBP1-89757 (A) shows similar ...read more
Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human testis.
Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human colon.
Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human lymph node using Anti-GOLGA2 antibody NBP1-89757.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GM130/GOLGA2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DTPKDNAATLQPSDDTVLPGGVPSPGASLTSMAASQNHDADNVPNLMDETKTFSSTESLRQLSQQLNGLVCESATCVNGEGPASSANLK
Marker
Golgi Apparatus Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLGA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GM130/GOLGA2 Protein (NBP1-89757PEP)
Publications
Read Publications using NBP1-89757.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24302887)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GM130/GOLGA2 Antibody

  • 130 kDa cis-Golgi matrix protein
  • GM130 autoantigen
  • GM130
  • GM130Golgi matrix protein GM130
  • GOLGA2
  • golgi autoantigen, golgin subfamily a, 2
  • golgin A2
  • Golgin subfamily A member 2
  • Golgin-95
  • MGC20672
  • SY11 Protein

Background

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GM130/GOLGA2 Antibody (NBP1-89757)(2)

We have publications tested in 3 applications: ICC/IF, Immunohistochemistry, Western Blot.


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ICC/IF
(1)
Immunohistochemistry
(1)
Western Blot
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GOLGA2