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GM130/GOLGA2 Antibody (SR1125)

Images

 
Immunohistochemistry: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using NBP3-21627.
Western Blot: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Western blot analysis using NBP3-21627 in (1) HeLa cell lysate; (2) MCF-7 cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
SR1125
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GM130/GOLGA2 Antibody (SR1125) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human GM130/GOLGA2 (Uniprot #: Q08379)
Specificity
Detects endogenous levels of total GM130/GOLGA2
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
GOLGA2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4), 150mM sodium chloride, 0.05% BSA, 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for GM130/GOLGA2 Antibody (SR1125)

  • 130 kDa cis-Golgi matrix protein
  • GM130 autoantigen
  • GM130
  • GM130Golgi matrix protein GM130
  • GOLGA2
  • golgi autoantigen, golgin subfamily a, 2
  • golgin A2
  • Golgin subfamily A member 2
  • Golgin-95
  • MGC20672
  • SY11 Protein

Background

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GOLGA2