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Glypican 3 Antibody (CL12711)

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Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Analysis in human placenta and skeletal muscle tissues using NBP3-18542 antibody. Corresponding GPC3 RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human liver cancer (hepatocellular carcinoma) shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human ovarian cancer (papillary carcinoma) shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human testis cancer (seminoma) shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human ovarian cancer (yolk sack tumor) shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: Glypican 3 Antibody (CL12711) [NBP3-18542] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clone
CL12711
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Glypican 3 Antibody (CL12711) Summary

Immunogen
This antibody was developed using a synthetic peptide derived from P51654, with the exact immunogen sequence remaining proprietary.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GPC3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 -1:2500
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Glypican 3 Antibody (CL12711)

  • DGSX
  • Glypican 3
  • glypican proteoglycan 3
  • glypican-3
  • GPC3
  • GTR2-2
  • heparan sulphate proteoglycan
  • Intestinal protein OCI-5
  • MXR7
  • OCI5
  • OCI-5
  • secreted glypican-3
  • SGB
  • SGBS
  • SGBS1SDYS

Background

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Blogs on Glypican 3.

Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma
By Jamshed Arslan, Pharm. D., PhD. Gastroesophageal adenocarcinoma originates from the glandular epithelium of the esophagus, gastroesophageal junction and stomach. The incidence of gastroesophageal adenocarcinoma is ...  Read full blog post.

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Bioinformatics

Gene Symbol GPC3