Novus Biologicals products are now on bio-techne.com

Glutathione Peroxidase 4/GPX4 Antibody

Images

 
Western Blot: Glutathione Peroxidase 4/GPX4 Antibody [NBP1-54691] - 25 ug of indicated human cell extracts were loaded onto a 10% - 20% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended ...read more
Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody [NBP1-54691] - Human Heart Tissue, antibody concentration 5 ug/ml.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Glutathione Peroxidase 4/GPX4 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to GPX4(glutathione peroxidase 4 (phospholipid hydroperoxidase)) The peptide sequence was selected from the middle region of GPX4. Peptide sequence QUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAA. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPX4
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml
Application Notes
It is recommended to stain paraffin-embedded tissue samples with the antibody at 5ug/ml after heat-induced antigen retrieval with Sodium citrate buffer.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Glutathione Peroxidase 4/GPX4 Antibody

  • EC 1.11.1
  • EC 1.11.1.12
  • glutathione peroxidase 4 (phospholipid hydroperoxidase)
  • Glutathione Peroxidase 4
  • GPX4
  • GPx-4
  • GSHPx-4
  • MCSP
  • PHGPx
  • PHGPxsnGPx
  • phospholipid hydroperoxidase
  • phospholipid hydroperoxide glutathione peroxidase, mitochondrial
  • snGPx
  • snPHGPx
  • sperm nucleus glutathione peroxidase

Background

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3798
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NB100-2688
Species: Hu
Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, WB
NBP2-24915
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-16684
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-06398
Species: Hu, Pm, Rt
Applications: IB, ICC/IF, IHC, IHC-P, WB
NBP1-32002
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB100-1992
Species: Bv, Ca, Ch, Dr, Gt, Gp, Ha, Hu, Pm, Mu, Po, Rb, Rt, Sh, Sq, Xp
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
MAB7428
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ICFlow, WB
NBP2-37253
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP1-86616
Species: Hu
Applications: IHC, IHC-P, WB
AF5724
Species: Hu, Mu, Rt
Applications: ICC, WB
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-94256
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-90338
Species: Hu
Applications: IHC, IHC-P
NBP2-52983
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-54691
Species: Hu, Rt
Applications: WB, IHC

Publications for Glutathione Peroxidase 4/GPX4 Antibody (NBP1-54691) (0)

There are no publications for Glutathione Peroxidase 4/GPX4 Antibody (NBP1-54691).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Glutathione Peroxidase 4/GPX4 Antibody (NBP1-54691) (0)

There are no reviews for Glutathione Peroxidase 4/GPX4 Antibody (NBP1-54691). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Glutathione Peroxidase 4/GPX4 Antibody (NBP1-54691) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Glutathione Peroxidase 4/GPX4 Products

Research Areas for Glutathione Peroxidase 4/GPX4 Antibody (NBP1-54691)

Find related products by research area.

Blogs on Glutathione Peroxidase 4/GPX4

There are no specific blogs for Glutathione Peroxidase 4/GPX4, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Glutathione Peroxidase 4/GPX4 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GPX4
Uniprot