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Glutathione Peroxidase 4/GPX4 Antibody (LHM 2)

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Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) [NBP3-07344] - Confocal immunofluorescence image of HepG2 cells stained with Glutathione Peroxidase 4/GPX4 Mouse Monoclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC
Clone
LHM 2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08253)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
A 375P cells crude extract
Localization
Mitochondrion, Cytoplasm
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GPX4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2)

  • EC 1.11.1
  • EC 1.11.1.12
  • glutathione peroxidase 4 (phospholipid hydroperoxidase)
  • Glutathione Peroxidase 4
  • GPX4
  • GPx-4
  • GSHPx-4
  • MCSP
  • PHGPx
  • PHGPxsnGPx
  • phospholipid hydroperoxidase
  • phospholipid hydroperoxide glutathione peroxidase, mitochondrial
  • snGPx
  • snPHGPx
  • sperm nucleus glutathione peroxidase

Background

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GPX4
Uniprot