![Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of GPX4 on Zebrafish tissue lysates using anti-GPX4 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Western-Blot-NBP2-75511-img0005.jpg "Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of GPX4 on Zebrafish tissue lysates using anti-GPX4 antibody at 1/500 dilution.")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | JU11-31 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Glutathione Peroxidase 4/GPX4 aa 23-72 / 197. (SwissProt: P36969 Human; SwissProt: O70325 Mouse; SwissProt: P36970 Rat) |
| Localization | Mitochondrion, Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GPX4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot Peroxiredoxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Peroxiredoxin_5_AF5724_Western_Blot_5819.jpg)
![Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Peroxiredoxin_5_AF5724_Immunocytochemistry__Immunofluorescence_19720.jpg)
![Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Peroxiredoxin_5_AF5724_Immunocytochemistry__Immunofluorescence_19954.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Glutathione Peroxidase 4/GPX4 Antibody (NBP2-75511)Find related products by research area.
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| Gene Symbol | GPX4 |
![Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of MCF-7 cells labeling GPX4 with Rabbit anti-Gluthathione Peroxidase/GPX4 antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GPX4 antibody. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunocytochemistry-Immunofluorescence-NBP2-75511-img0007.jpg "Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of MCF-7 cells labeling GPX4 with Rabbit anti-Gluthathione Peroxidase/GPX4 antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GPX4 antibody. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.")
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Glutathione Peroxidase 4/GPX4 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunohistochemistry-Paraffin-NBP2-75511-img0008.jpg "Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Glutathione Peroxidase 4/GPX4 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded human kidney tissue using anti-GPX4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunohistochemistry-Paraffin-NBP2-75511-img0001.jpg "Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded human kidney tissue using anti-GPX4 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded mouse testis tissue using anti-GPX4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunohistochemistry-Paraffin-NBP2-75511-img0002.jpg "Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded mouse testis tissue using anti-GPX4 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded rat epididymis tissue using anti-GPX4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-4-GPX4-Antibody-JU11-31-Immunohistochemistry-Paraffin-NBP2-75511-img0003.jpg "Immunohistochemistry-Paraffin: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - Analysis of paraffin-embedded rat epididymis tissue using anti-GPX4 antibody. Counter stained with hematoxylin.")
![Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody at 1/500 dilution.Lane 1: Hela-si NT cell lysateLane 2: Hela-si GPX4 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 22 kDaObserved band size: 20,22 kDaExposure time: 1 minutes 40 seconds;4-20% SDS-PAGE gel. was shown to specifically react with GPX4 in Hela-si NT cells. Weakened band was observed when Hela-si GPX4 sample was tested. Hela-si NT and Hela-si GPX4 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Rabbit anti-GAPDH , 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75511_rabbit-monoclonal-glutathione-peroxidase-4-gpx4-antibody-ju11-31-western-blot-187202483435..jpg "Western Blot: Glutathione Peroxidase 4/GPX4 Antibody (JU11-31) [NBP2-75511] - analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody at 1/500 dilution.Lane 1: Hela-si NT cell lysateLane 2: Hela-si GPX4 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 22 kDaObserved band size: 20,22 kDaExposure time: 1 minutes 40 seconds;4-20% SDS-PAGE gel. was shown to specifically react with GPX4 in Hela-si NT cells. Weakened band was observed when Hela-si GPX4 sample was tested. Hela-si NT and Hela-si GPX4 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Rabbit anti-GAPDH , 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Glutathione_Peroxidase_1_AF3798_Western_Blot_5348.jpg)
![Simple Western Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Glutathione_Peroxidase_1_AF3798_Simple_Western_17131.jpg)
![Immunohistochemistry Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Glutathione_Peroxidase_1_AF3798_Immunohistochemistry_6806.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")