![Western Blot: Glutathione Peroxidase 2/GPX2 Antibody (JE40-73) [NBP3-32390] - Western blot analysis of Glutathione Peroxidase 2/GPX2 on different lysates with Rabbit anti-Glutathione Peroxidase 2/GPX2 antibody (NBP3-32390) at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: LoVo cell lysate Lane 3: HT-29 cell lysate Lane 4: AGS cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32390) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32390_rabbit-glutathione-peroxidase-2-gpx2-mab-je40-73-278202416285249.jpg "Western Blot: Glutathione Peroxidase 2/GPX2 Antibody (JE40-73) [NBP3-32390] - Western blot analysis of Glutathione Peroxidase 2/GPX2 on different lysates with Rabbit anti-Glutathione Peroxidase 2/GPX2 antibody (NBP3-32390) at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: LoVo cell lysate Lane 3: HT-29 cell lysate Lane 4: AGS cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32390) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clone | JE40-73 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within C terminal Human Glutathione Peroxidase 2/GPX2. (Uniprot: P18283) |
| Localization | Cytoplasm, cytosol. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GPX2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Glutathione Peroxidase 2/GPX2 Antibody (NBP3-32390)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GPX2 |
![Western Blot Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Glutathione_Peroxidase_1_AF3798_Western_Blot_5348.jpg)
![Simple Western Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Glutathione_Peroxidase_1_AF3798_Simple_Western_17131.jpg)
![Immunohistochemistry Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Glutathione_Peroxidase_1_AF3798_Immunohistochemistry_6806.jpg)
![Western Blot Glutathione Peroxidase 4/GPX4 Antibody (565320) [Unconjugated]](https://images.novusbio.com/images2/Glutathione_Peroxidase_4_MAB5457_Western_Blot_6237.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Western Blot Keap1 Antibody (333116) [Unconjugated]](https://images.novusbio.com/images2/Keap1_MAB3024_Western_Blot_6061.jpg)
![Simple Western Keap1 Antibody (333116) [Unconjugated]](https://images.novusbio.com/images2/Keap1_MAB3024_Simple_Western_16632.jpg)
![Knockout Validated Keap1 Antibody (333116) [Unconjugated]](https://images.novusbio.com/images2/Keap1_MAB3024_Knockout_Validated_23684.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")