Glutathione Peroxidase 4/GPX4 Antibody (565320) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Glutathione Peroxidase 4 (GPX4; also known as PHGPx) is a monomeric, 21 kDa member of the glutathione peroxidase family of proteins. It is widely expressed and serves to both protect cell membranes from phospholipid and cholesterol hydroperoxidases, and form an inactive enzyme structural component of the sperm mitochondrial capsule. Human GPX4 is 197 amino acids (aa) in length and contains a mitochondrial targeting sequence (aa 1‑27) and an enzymatically active selenocysteine at Sec73. During sperm maturation, the targeting sequence is cleaved, and GPX4 may form inactive covalently-linked oligomers. There are multiple splice variants. One is ubiquitously expressed, cytoplasmic, and shows an alternate start site at Met28. A second is 226 aa in length, nuclear, and shows an Arg-rich 65 aa substitution for aa 1‑28. Over amino acids 74‑168, human GPX4 shares 93% aa identity with mouse GPX4.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: Western Blot.

Showing Publications 1 - 10 of 23. Show All 23 Publications. Collapse Publications.

Publications using MAB5457	Applications	Species
Lei, G;Mao, C;Horbath, AD;Yan, Y;Cai, S;Yao, J;Jiang, Y;Sun, M;Liu, X;Cheng, J;Xu, Z;Lee, H;Li, Q;Lu, Z;Zhuang, L;Chen, MK;Alapati, A;Yap, TA;Hung, MC;You, MJ;Piwnica-Worms, H;Gan, B; BRCA1-mediated dual regulation of ferroptosis exposes a vulnerability to GPX4 and PARP co-inhibition in BRCA1-deficient cancers Cancer discovery 2024-03-28 [PMID: 38552003] (Western Blot, Human)	Western Blot	Human
Chen, Y;Guo, X;Zeng, Y;Mo, X;Hong, S;He, H;Li, J;Fatima, S;Liu, Q; Oxidative stress induces mitochondrial iron overload and ferroptotic cell death Scientific reports 2023-09-19 [PMID: 37726294] (Western Blot, Rat)	Western Blot	Rat
Kose, T;Moreno-Fernandez, J;Vera-Aviles, M;Sharp, PA;Latunde-Dada, GO; Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage Biochemistry and biophysics reports 2023-09-01 [PMID: 37560439] (Western Blot, Mouse)	Western Blot	Mouse
Enrico Radaelli, Charles-Antoine Assenmacher, Jillian Verrelle, Esha Banerjee, Florence Manero, Salim Khiati et al. Mitochondrial defects leading to arrested spermatogenesis and ferroptosis in the PARL deficient mouse model of Leigh Syndrome eLife 2023-07-28 [PMID: 37505079]
Mao, C;Liu, X;Yan, Y;Olszewski, K;Gan, B; Reply to: DHODH inhibitors sensitize to ferroptosis by FSP1 inhibition Nature 2023-07-01 [PMID: 37407682] (Western Blot, Human)	Western Blot	Human
J Liu, S Lane, R Lall, M Russo, L Farrell, M Debreli Co, C Curtin, R Araujo-Gut, M Scherrer-C, BH Trachtenbe, J Kim, E Tolosano, A Ghigo, RE Gerszten, A Asnani Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice Science Advances, 2022-12-23;8(51):eadc9245. 2022-12-23 [PMID: 36563141] (Western Blot, Mouse)	Western Blot	Mouse
T Kose, PA Sharp, GO Latunde-Da Upregulation of Nrf2 Signalling and the Inhibition of Erastin-Induced Ferroptosis by Ferulic Acid in MIN6 Cells International Journal of Molecular Sciences, 2022-12-14;23(24):. 2022-12-14 [PMID: 36555529] (Western Blot, Mouse)	Western Blot	Mouse
Zelong Gao, Dongyao Wang, Hongwei Zhang, Jianxin Yang, Min Li, Hongtao Lu et al. An iron-deficient diet prevents alcohol- or diethylnitrosamine-induced acute hepatotoxicity in mice by inhibiting ferroptosis Current Research in Food Science 2022-11-05 [PMID: 36387594]
LB Pontel, A Bueno-Cost, AE Morellato, J Carvalho S, G Roué, M Esteller Acute lymphoblastic leukemia necessitates GSH-dependent ferroptosis defenses to overcome FSP1-epigenetic silencing Redox Biology, 2022-07-31;55(0):102408. 2022-07-31 [PMID: 35944469] (Western Blot, Human)	Western Blot	Human
Shiqi Wu, Chao Mao, Lavanya Kondiparthi, Masha V. Poyurovsky, Kellen Olszewski, Boyi Gan A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria Proceedings of the National Academy of Sciences 2022-06-28 [PMID: 35749365]
Wenli Liu, Nataliya Östberg, Mustafa Yalcinkaya, Huijuan Dou, Kaori Endo-Umeda, Yang Tang et al. Erythroid lineage Jak2V617F expression promotes atherosclerosis through erythrophagocytosis and macrophage ferroptosis Journal of Clinical Investigation 2022-07-01 [PMID: 35587375]
P Koppula, G Lei, Y Zhang, Y Yan, C Mao, L Kondiparth, J Shi, X Liu, A Horbath, M Das, W Li, MV Poyurovsky, K Olszewski, B Gan A targetable CoQ-FSP1 axis drives ferroptosis- and radiation-resistance in KEAP1 inactive lung cancers Nature Communications, 2022-04-22;13(1):2206. 2022-04-22 [PMID: 35459868] (Western Blot, Human)	Western Blot	Human
F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis Nature Communications, 2021-12-17;12(1):7333. 2021-12-17 [PMID: 34921145] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
Karmi O, Sohn YS, Zandalinas SI et al. Disrupting CISD2 function in cancer cells primarily impacts mitochondrial labile iron levels and triggers TXNIP expression Free Radical Biology and Medicine 2021-11-01 [PMID: 34547371]
Ola Karmi, Yang-Sung Sohn, Henri-Baptiste Marjault, Tal Israeli, Gil Leibowitz, Konstantinos Ioannidis et al. A Combined Drug Treatment That Reduces Mitochondrial Iron and Reactive Oxygen Levels Recovers Insulin Secretion in NAF-1-Deficient Pancreatic Cells Antioxidants (Basel) 2021-07-21 [PMID: 34439408]
C Mao, X Liu, Y Zhang, G Lei, Y Yan, H Lee, P Koppula, S Wu, L Zhuang, B Fang, MV Poyurovsky, K Olszewski, B Gan DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer Nature, 2021-05-12;0(0):. 2021-05-12 [PMID: 33981038] (Western Blot, Human)	Western Blot	Human
Y Zhang, RV Swanda, L Nie, X Liu, C Wang, H Lee, G Lei, C Mao, P Koppula, W Cheng, J Zhang, Z Xiao, L Zhuang, B Fang, J Chen, SB Qian, B Gan mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation Nature Communications, 2021-03-11;12(1):1589. 2021-03-11 [PMID: 33707434] (Western Blot, Human)	Western Blot	Human
H Lee, F Zandkarimi, Y Zhang, JK Meena, J Kim, L Zhuang, S Tyagi, L Ma, TF Westbrook, GR Steinberg, D Nakada, BR Stockwell, B Gan Energy-stress-mediated AMPK activation inhibits ferroptosis Nat. Cell Biol., 2020-02-06;22(2):225-234. 2020-02-06 [PMID: 32029897] (Western Blot, Human)	Western Blot	Human
Guang Lei, Yilei Zhang, Pranavi Koppula, Xiaoguang Liu, Jie Zhang, Steven H. Lin et al. The role of ferroptosis in ionizing radiation-induced cell death and tumor suppression Cell Research 2020-02-01 [PMID: 31949285]
A Gomaa, D Peng, Z Chen, M Soutto, K Abouelezz, A Corvalan, W El-Rifai Epigenetic regulation of AURKA by miR-4715-3p in upper gastrointestinal cancers Sci Rep, 2019-11-18;9(1):16970. 2019-11-18 [PMID: 31740746] (Western Blot, Human)	Western Blot	Human
Che‐Lin Hu, Mara Nydes, Kara L. Shanley, Itzy E. Morales Morales Pantoja, Tamara A. Howard, Oscar A. Bizzozero Reduced expression of the ferroptosis inhibitor glutathione peroxidase‐4 in multiple sclerosis and experimental autoimmune encephalomyelitis Journal of Neurochemistry 2019-02-01 [PMID: 30289974]
KJ Habermann, L Grünewald, S van Wijk, S Fulda Targeting redox homeostasis in rhabdomyosarcoma cells: GSH-depleting agents enhance auranofin-induced cell death Cell Death Dis, 2017-10-05;8(10):e3067. 2017-10-05 [PMID: 28981107] (Western Blot, Human)	Western Blot	Human
Schoeneberger H, Belz K, Schenk B, Fulda S Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death. Oncogene, 2014-11-10;34(31):4032-43. 2014-11-10 [PMID: 25381820] (Western Blot, Human)	Western Blot	Human
Show All 23 Publications. Collapse Publications.

Array MAB5457

• Glutathione Peroxidase 4/GPX4 Antibodies
• Glutathione Peroxidase 4/GPX4 ELISA Kits
• Glutathione Peroxidase 4/GPX4 Lysate
• Glutathione Peroxidase 4/GPX4 Proteins