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Glutathione Peroxidase 2/GPX2 Antibody (496010)

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, Hepa 1-6 mouse hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 2 Monoclonal Antibody (Catalog # MAB5470) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, Hepa 1-6 mouse hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ...read more
Expression of various ROS-related proteins, pro-inflammatory factors, and an osteoclast differentiation factor RANKL in tissue samples of all treated groups at 24 weeks after surgery.(A) Representative Western blot ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
496010
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5470-SP
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Glutathione Peroxidase 2/GPX2 Antibody (496010) Summary

Immunogen
E. coli-derived recombinant human Glutathione Peroxidase 2
Gly41-Ile190
Accession # P18283
Specificity
Detects endogenous human, mouse and rat GPX2 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GPX2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB5470 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutathione Peroxidase 2/GPX2 Antibody (496010)

  • EC 1.11.1
  • EC 1.11.1.9
  • gastrointestinal glutathione peroxidase 2
  • Gastrointestinal glutathione peroxidase
  • GIGPX
  • GI-GPx
  • glutathione peroxidase 2 (gastrointestinal)
  • Glutathione Peroxidase 2
  • Glutathione peroxidase-gastrointestinal
  • Glutathione peroxidase-related protein 2
  • GPRP
  • GPRP-2
  • GPX2
  • GPx-2
  • GPx-GI
  • GSHPx-2
  • GSHPX-GI

Background

Glutathione Peroxidase 2 (GPX2), also known as Gastrointestinal Glutathione Peroxidase, is a member of the glutathione peroxidase antioxidant enzyme family. These proteins protect cell surfaces, extracellular fluid components, and enzymes from oxidative stress by reducing hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX2 is a cytoplasmic homotetramer of 22 kDa subunits, each containing a selenocysteine residue at the active site. GPX2 is primarily expressed in the liver and intestinal epithelial cells where it participates in the detoxification of dietary organic hydroperoxides. It is upregulated in a variety of carcinomas and functions as an anti-inflammatory and anti-apoptotic molecule. Over amino acids 41‑190, human GPX2 shares 94% aa sequence identity with mouse and rat GPX2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutathione Peroxidase 2/GPX2 Antibody (MAB5470)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: Western Blot.


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(2)
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Bioinformatics

Gene Symbol GPX2
Uniprot