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Glucuronic Acid Epimerase Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Glucuronic Acid Epimerase Antibody [NBP2-31985] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Glucuronic Acid Epimerase Antibody [NBP2-31985] - Staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.

Product Details

Summary
Reactivity Hu, Eq, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Glucuronic Acid Epimerase Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SDKAIQFPRRSSSGFRVDGFEKRAAASESNNYMNHVAKQQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDR
Predicted Species
Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLCE
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Glucuronic Acid Epimerase Protein (NBP2-31985PEP)
Publications
Read Publication using
NBP2-31985 in the following applications:

Reactivity Notes

Equine reactivity reported in the literature (PMID: 30227887).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Glucuronic Acid Epimerase Antibody

  • D-glucuronyl C5-epimerase
  • EC 5.1.3
  • EC 5.1.3.-
  • glucuronic acid epimerase
  • glucuronyl C5-epimerase
  • Heparin/heparan sulfate:glucuronic acid C5-epimerase
  • HSEPIheparin/heparan sulfate-glucuronic acid C5-epimerase
  • KIAA0836heparan sulfate epimerase
  • UDP-glucuronic acid epimerase

Background

Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS, which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glucuronic Acid Epimerase Antibody (NBP2-31985)(1)

We have publications tested in 1 confirmed species: Equine.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
Equine
(1)
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GLCE
Uniprot