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GDF-5/BMP-14 Antibody (90509) [Biotin]

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GDF-5 was detected in immersion fixed frozen sections of mouse embryo using Mouse GDF-5 Biotinylated Monoclonal Antibody (Catalog # BAM853) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
GDF-5 was detected in immersion fixed frozen sections of mouse embryo using Mouse GDF-5 Biotinylated Monoclonal Antibody (Catalog # BAM853) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clone
90509
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
MAB853
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB853
Carrier Free
MAB853
Carrier Free
MAB853-SP
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GDF-5/BMP-14 Antibody (90509) [Biotin] Summary

Immunogen
E. coli-derived recombinant mouse GDF‑5/BMP‑14
Ala376-Arg495
Accession # P43027
Specificity
Detects mouse GDF‑5/BMP‑14 in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
GDF5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-5/BMP-14 Antibody (90509) [Biotin]

  • BMP14
  • BMP-14
  • Cartilage-derived morphogenetic protein 1
  • cartilage-derived morphogenetic protein-1
  • CDMP-1
  • CDMP1LAP4
  • GDF5
  • GDF-5
  • growth differentiation factor 5
  • growth/differentiation factor 5
  • OS5
  • radotermin
  • SYNS2

Background

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6, and GDF-7, which share 80-86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
  5. Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
  6. Inada, M. et al. (1996) BBRC 222:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GDF-5/BMP-14
Antibody (90509) [Bio
BAM853
GDF-5/BMP-14 Antibody (90509) [Biotin]
Species: Mu
Applications: WB, IHC

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Video Protocols

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Bioinformatics

Gene Symbol GDF5
Uniprot