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GCLC Antibody (0X1H3)

Images

 
Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of Rat kidney, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more
Immunohistochemistry-Paraffin: GCLC Antibody (0X1H3) [NBP3-16422] - Rat kidney using GCLC Rabbit mAb (NBP3-16422) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before ...read more
Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of various cell lines, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC
Clone
0X1H3
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GCLC Antibody (0X1H3) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 538-637 of human GCLC (P48506). LNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
GCLC
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for GCLC Antibody (0X1H3)

  • EC 6.3.2.2
  • Gamma-ECS
  • Gamma-glutamylcysteine synthetase
  • GCLC
  • GCS heavy chain
  • GCS
  • GLCL
  • GLCLC
  • GLCLCGCL
  • glutamate--cysteine ligase catalytic subunit
  • glutamate-cysteine ligase, catalytic subunit

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GCLC Antibody (NBP3-16422) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GCLC Antibody (NBP3-16422) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for GCLC Antibody (NBP3-16422)

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Blogs on GCLC.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

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Bioinformatics

Gene Symbol GCLC