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GCAT Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GCAT Antibody [NBP1-81564] - Staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria.
Immunohistochemistry: GCAT Antibody [NBP1-81564] - Staining of human pancreas shows moderate cytoplasmic positivity in with a granular pattern in exocrine and endocrine cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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GCAT Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKMEAAGFTISGA
Predicted Species
Mouse (91%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GCAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GCAT Protein (NBP1-81564PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GCAT Antibody

  • 2-amino-3-ketobutyrate-CoA ligase
  • AKB ligase
  • Aminoacetone synthase
  • EC 2.3.1
  • EC 2.3.1.29
  • Glycine acetyltransferase
  • glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase)
  • glycine C-acetyltransferase
  • KBL2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
  • MGC23053

Background

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threoninedehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrateby L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reactionbetween 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a classII pyridoxal-phosphate-dependent aminotransferase. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for GCAT Antibody (NBP1-81564) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GCAT