![Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-322-img0005.jpg "Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.")
| Reactivity | Hu, Mu, Rt, Ch, Pm, Po, Am, Av, Bv, Ca, ChHa, Ma, Fi, SyHa, Gp, Ha, Rb, Sh, Sq, Tr, Xp, ZeSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, IP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | This GAPDH antibody was developed against an epitope between residues 150 and 200 of human GAPDH using the numbering given in entry NP_002037.2 (GeneID 2597). |
| Marker | Cytosolic Marker |
| Predicted Species | Porcine (100%), Ferret (100%), Bovine (100%), Guinea Pig (100%), Squirrel (100%), Rabbit (100%), Zebrafish (100%), Amphibian (100%), Fish (100%), Avian (100%), Turkey (100%), Xenopus (100%), Chinese Hamster (100%), Canine (100%), Hamster (100%), Sheep (100%), Golden Syrian Hamster (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GAPDH |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This GAPDH antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin applications. For IHC, antigen retrieval with citrate buffer pH6.0 is recommended for formalin fixed paraffin embedded tissue sections. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
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reviewed by:
Verified Customer |
WB | Mouse | 02/28/2014 |
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reviewed by:
Verified Customer |
WB | Human | 09/29/2011 |
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Secondary Antibodies |
Isotype Controls |
Research Areas for GAPDH Antibody (NB300-322)Find related products by research area.
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Considerations for Quantitative Western blotting By Jamshed Arslan, Pharm. D., PhD. Since its inception in 1979, Western blotting has undergone several developments. The use of radioactive probes was common throughout 1980s, but utilizing secondary antibodies labell... Read full blog post. |
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The use of Beta Actin (AC-15) as a loading control across multiple species Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post. |
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Tips on choosing an ideal loading control antibody for Western Blotting Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p... Read full blog post. |
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GAPDH - A "Housekeeping" Gene With Diverse Functions in Cellular Homeostasis Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing western blots. However, this ... Read full blog post. |
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A New Standard in Antibody Testing - Simple Western Certified Antibodies The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi... Read full blog post. |
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GAPDH: More than a housekeeping gene GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway. Apart from its role in gl... Read full blog post. |
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GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase) GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin... Read full blog post. |
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Time to Start Actin Like a Reliable 'Housekeeper'! A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti... Read full blog post. |
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Beta Actin and GAPDH: The Importance of Western Blot Loading Controls What are Western Blot Loading Controls?Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use... Read full blog post. |
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The GAPDH Antibody in Western Blot Assays The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Application: | WB | |
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| Application: | WB | |
| Species: | Human |
![Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-322 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0007.jpg "Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-322 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 ug) and human HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0006.jpg "Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 ug) and human HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-322-img0008.jpg "Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB")
![Western Blot: GAPDH Antibody [NB300-322] - c-Src is required for efficient translation of PRC2 component mRNAs. Immunoblot of PRC2 components in control and c-Src-deficient tumors. Bar chart shows quantification of fluorescent immunoblot data normalized to the loading control (Gapdh). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10681-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0011.jpg "Western Blot: GAPDH Antibody [NB300-322] - c-Src is required for efficient translation of PRC2 component mRNAs. Immunoblot of PRC2 components in control and c-Src-deficient tumors. Bar chart shows quantification of fluorescent immunoblot data normalized to the loading control (Gapdh). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10681-4), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-322] - GAPDH detection in HeLa cells with ICC-IF application using NB300-322, visualized with DyLight Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-322-img0003.jpg "Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-322] - GAPDH detection in HeLa cells with ICC-IF application using NB300-322, visualized with DyLight Fluor 488.")
![Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-322-img0009.jpg "Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB")
![Western Blot: GAPDH Antibody [NB300-322] - Mouse DRG stained at 1:2000 dilution. Image provided by verified customer review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0004.jpg "Western Blot: GAPDH Antibody [NB300-322] - Mouse DRG stained at 1:2000 dilution. Image provided by verified customer review.")
![Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis and chemotaxis. Western blot analyses were performed on 70 ug of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1:2,000) or anti-GAPDH (1:1,000) antibody. ***: significantly different, p<0.0001, Student's t-test. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917) licensed under a CC-BY license](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0010.jpg "Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis and chemotaxis. Western blot analyses were performed on 70 ug of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1:2,000) or anti-GAPDH (1:1,000) antibody. ***: significantly different, p<0.0001, Student's t-test. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917) licensed under a CC-BY license")
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of human lung carcinoma using NB300-322 at a dilution of 1:200.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-322-img0001.jpg "Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of human lung carcinoma using NB300-322 at a dilution of 1:200.")
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of mouse squamous cell carcinoma using NB300-322 at a dilution of 1:200.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-322-img0002.jpg "Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of mouse squamous cell carcinoma using NB300-322 at a dilution of 1:200.")
![Western Blot: GAPDH Antibody [NB300-322] - Concentration-dependent effects of subchronic administration of ZTP on Cx43 protein expression in the plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Concentration-dependent effects of ZTP (100, 300 & 1000 nM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by one-way ANOVA with Tukey’s (wholly significant difference) post hoc test (** p < 0.01 vs. ZTP free (0)). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-31020241528457.jpg "Western Blot: GAPDH Antibody [NB300-322] - Concentration-dependent effects of subchronic administration of ZTP on Cx43 protein expression in the plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Concentration-dependent effects of ZTP (100, 300 & 1000 nM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by one-way ANOVA with Tukey’s (wholly significant difference) post hoc test (** p < 0.01 vs. ZTP free (0)). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody [NB300-322] - Concentration-dependent effects of subchronic administration of ZTP on Cx43 protein expression in the cytosol fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Concentration-dependent effects of ZTP (100, 300 & 1000 nM) on Cx43 expression in the cytosol fraction of the primary cultured astrocytes were analysed by one-way ANOVA with Tukey’s (wholly significant difference) post hoc test (* p < 0.05 vs. ZTP free (0)). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-31020241536379.jpg "Western Blot: GAPDH Antibody [NB300-322] - Concentration-dependent effects of subchronic administration of ZTP on Cx43 protein expression in the cytosol fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Concentration-dependent effects of ZTP (100, 300 & 1000 nM) on Cx43 expression in the cytosol fraction of the primary cultured astrocytes were analysed by one-way ANOVA with Tukey’s (wholly significant difference) post hoc test (* p < 0.05 vs. ZTP free (0)). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody [NB300-322] - Effects of acute (120 min) administration of supratherapeutic concentration of ZTP (1000 nM) & chronic (14 days) administration of therapeutically relevant concentrations of ZTP (300 nM) on Cx43 protein expression in the plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of ZTP on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by student T-test (* p < 0.05 vs. control: ZTP free). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-31020241534374.jpg "Western Blot: GAPDH Antibody [NB300-322] - Effects of acute (120 min) administration of supratherapeutic concentration of ZTP (1000 nM) & chronic (14 days) administration of therapeutically relevant concentrations of ZTP (300 nM) on Cx43 protein expression in the plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of ZTP on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by student T-test (* p < 0.05 vs. control: ZTP free). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody [NB300-322] - Interaction between subchronic administration of supratherapeutic concentration of ZTP & Akt inhibitor (DEBC) on Cx43 protein expression in the astroglial plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of ZTP (1000 nM) & Akt inhibitor (DEBC: 10 μM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by two-way ANOVA with Tukey’s (wholly significant difference) post hoc test (** p < 0.01 vs. control, @@ p < 0.01 vs. non). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-310202415371937.jpg "Western Blot: GAPDH Antibody [NB300-322] - Interaction between subchronic administration of supratherapeutic concentration of ZTP & Akt inhibitor (DEBC) on Cx43 protein expression in the astroglial plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of ZTP (1000 nM) & Akt inhibitor (DEBC: 10 μM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by two-way ANOVA with Tukey’s (wholly significant difference) post hoc test (** p < 0.01 vs. control, @@ p < 0.01 vs. non). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Simple Western: GAPDH Antibody [NB300-322] - Effects of subchronic nicotine administration on the expression of phosphorylated protein kinase B (pAkt) & phosphorylated extracellular signal-regulated kinase (pErk) in the plasma membrane fraction of OFC. Effects of the systemic subchronic administration of nicotine (50 mg/kg/day for seven days) on pAkt & pErk expression in the OFC plasma membrane fraction before four week of age (A,C) & after 12 week of age (B,D), ADSHE onset of the wild-type & S286L-TG & pseudo-gel images, using capillary immunoblotting. Ordinate: mean ± SD (n = 6) of the relative protein level of pErk & pAkt. * p < 0.05, ** p < 0.01 vs. wild-type, & @ p < 0.05, @@ p < 0.01 vs. nicotine-free (non) by two-way ANOVA with Tukey’s multiple comparison. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33143372), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-310202415175247.jpg "Simple Western: GAPDH Antibody [NB300-322] - Effects of subchronic nicotine administration on the expression of phosphorylated protein kinase B (pAkt) & phosphorylated extracellular signal-regulated kinase (pErk) in the plasma membrane fraction of OFC. Effects of the systemic subchronic administration of nicotine (50 mg/kg/day for seven days) on pAkt & pErk expression in the OFC plasma membrane fraction before four week of age (A,C) & after 12 week of age (B,D), ADSHE onset of the wild-type & S286L-TG & pseudo-gel images, using capillary immunoblotting. Ordinate: mean ± SD (n = 6) of the relative protein level of pErk & pAkt. * p < 0.05, ** p < 0.01 vs. wild-type, & @ p < 0.05, @@ p < 0.01 vs. nicotine-free (non) by two-way ANOVA with Tukey’s multiple comparison. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33143372), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Simple Western: GAPDH Antibody [NB300-322] - Effects of the subacute administration of effective doses of vortioxetine (2.5 mg/kg/day), escitalopram (5 mg/kg/day), & lurasidone (3 mg/kg/day) for 3 days on the expression of 5-HT7R in the thalamic plasma membrane fraction (Panel (A)). Ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R in the thalamic plasma membrane fraction. Panel (B) indicates the pseudo-gel images using capillary immunoblotting. * p < 0.05, ** p < 0.01 vs. the control by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33572981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-310202415304278.jpg "Simple Western: GAPDH Antibody [NB300-322] - Effects of the subacute administration of effective doses of vortioxetine (2.5 mg/kg/day), escitalopram (5 mg/kg/day), & lurasidone (3 mg/kg/day) for 3 days on the expression of 5-HT7R in the thalamic plasma membrane fraction (Panel (A)). Ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R in the thalamic plasma membrane fraction. Panel (B) indicates the pseudo-gel images using capillary immunoblotting. * p < 0.05, ** p < 0.01 vs. the control by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33572981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase cell motility.(A): HeLa cells were transduced with Lenti-control or Lenti-Tax viral particles. When cells reached 100% confluence, a wound was made in the cell monolayer & pictures were taken at 0 h & up to 12 h post-wounding. Measurements between the 2 fronts of migration were performed using image-J software (NIH, USA). (B): Percentage of healing during a 12 h kinetic. (C): Western blot analyses were performed on 70 µg of cellular extracts transduced by Lenti-control or Lenti-Tax viral particles. Membranes were probed with anti-Gem (1∶2,000) or anti-GAPDH (1∶1,000) antibody. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-310202415291943.jpg "Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase cell motility.(A): HeLa cells were transduced with Lenti-control or Lenti-Tax viral particles. When cells reached 100% confluence, a wound was made in the cell monolayer & pictures were taken at 0 h & up to 12 h post-wounding. Measurements between the 2 fronts of migration were performed using image-J software (NIH, USA). (B): Percentage of healing during a 12 h kinetic. (C): Western blot analyses were performed on 70 µg of cellular extracts transduced by Lenti-control or Lenti-Tax viral particles. Membranes were probed with anti-Gem (1∶2,000) or anti-GAPDH (1∶1,000) antibody. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis & chemotaxis.(A, C): MOLT4 cells were transduced with Lenti-control or Lenti-GEM. Forty-eight hours later, 5.105 cells were collected & loaded on a 5 µm permeable Transwell filter in absence or presence of SDF1/CXCL12 (150 ng/ml). Twenty-four hours later, cell migration was quantified by flow cytometry (flow cytometer Facscalibur4c+HTS (BD biosciences)). (B, D): Western blot analyses were performed on 70 µg of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1∶2,000) or anti-GAPDH (1∶1,000) antibody. ***: significantly different, p<0.0001, Student's t-test. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-310202415291955.jpg "Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis & chemotaxis.(A, C): MOLT4 cells were transduced with Lenti-control or Lenti-GEM. Forty-eight hours later, 5.105 cells were collected & loaded on a 5 µm permeable Transwell filter in absence or presence of SDF1/CXCL12 (150 ng/ml). Twenty-four hours later, cell migration was quantified by flow cytometry (flow cytometer Facscalibur4c+HTS (BD biosciences)). (B, D): Western blot analyses were performed on 70 µg of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1∶2,000) or anti-GAPDH (1∶1,000) antibody. ***: significantly different, p<0.0001, Student's t-test. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody [NB300-322] - Effects of subchronic administration of therapeutic-relevant concentration of antipsychotics, CLZ (3 μM), QTP (1 μM) & BPZ (0.3 μM), on Cx43 protein expression in the plasma fraction (A) & their pseudo-gel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34070699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-31020241528459.jpg "Western Blot: GAPDH Antibody [NB300-322] - Effects of subchronic administration of therapeutic-relevant concentration of antipsychotics, CLZ (3 μM), QTP (1 μM) & BPZ (0.3 μM), on Cx43 protein expression in the plasma fraction (A) & their pseudo-gel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34070699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody [NB300-322] - Interaction between acute administration of therapeutic-relevant concentration of antipsychotics & Akt inhibitor (DEBC) on Cx43 protein expression in the astroglial plasma membrane fraction, after subchronic administration of therapeutic-relevant concentration of VPA (500 μM) (A). Interaction between subchronic administration of therapeutic-relevant concentration of antipsychotics & DEBC on Cx43 protein expression in the astroglial plasma membrane fraction, after subchronic administration of therapeutic-relevant concentration of VPA (B). Lower panels indicate their pseudo-gel images, using capillary immunoblotting. Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of antipsychotics & Akt inhibitor (DEBC: 10 μM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analyzed by MANOVA with Tukey’s post hoc test (** p < 0.01 vs. non, @@ p < 0.01 vs. control). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34070699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-310202416212310.jpg "Western Blot: GAPDH Antibody [NB300-322] - Interaction between acute administration of therapeutic-relevant concentration of antipsychotics & Akt inhibitor (DEBC) on Cx43 protein expression in the astroglial plasma membrane fraction, after subchronic administration of therapeutic-relevant concentration of VPA (500 μM) (A). Interaction between subchronic administration of therapeutic-relevant concentration of antipsychotics & DEBC on Cx43 protein expression in the astroglial plasma membrane fraction, after subchronic administration of therapeutic-relevant concentration of VPA (B). Lower panels indicate their pseudo-gel images, using capillary immunoblotting. Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of antipsychotics & Akt inhibitor (DEBC: 10 μM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analyzed by MANOVA with Tukey’s post hoc test (** p < 0.01 vs. non, @@ p < 0.01 vs. control). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34070699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody [NB300-322] - Gem is overexpressed in T- & non-T-Tax-expressing cells as well as in HTLV-1 infected cells.(A): Total RNA was extracted from 293T cells transduced with Lenti-control (lane 2) or Lenti-Tax (lane 3) & RT-PCR was performed using gem or GADPH specific primers. Lane 1 is a control of extraction. (B, C): Western blot analyses were performed with 70 µg of proteins from (B) 293T or (C) MOLT4 cells transduced by Lenti-control or Lenti-Tax vectors (72 h post-transduction). (D): JPX-9 cells were grown for 24 h or 48 h in the presence of ZnCl2 (120 µM). Western blot analyses were performed with 70 µg of JPX-9 cellular extracts. (E): Western blot analyses were performed with 70 µg of cellular extracts obtained from non-infected (CEM, Jurkat & MOLT4) or HTLV-1-infected (C8166, C91/PL, Hut102 & MT2) T-lymphocytes. (B, C, D, E): Membranes were probed with anti-Gem (1∶2,000) & anti-GAPDH (1∶1,000) or anti-Tax Tab 172 (1∶500) antibodies. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-310202416212366.jpg "Western Blot: GAPDH Antibody [NB300-322] - Gem is overexpressed in T- & non-T-Tax-expressing cells as well as in HTLV-1 infected cells.(A): Total RNA was extracted from 293T cells transduced with Lenti-control (lane 2) or Lenti-Tax (lane 3) & RT-PCR was performed using gem or GADPH specific primers. Lane 1 is a control of extraction. (B, C): Western blot analyses were performed with 70 µg of proteins from (B) 293T or (C) MOLT4 cells transduced by Lenti-control or Lenti-Tax vectors (72 h post-transduction). (D): JPX-9 cells were grown for 24 h or 48 h in the presence of ZnCl2 (120 µM). Western blot analyses were performed with 70 µg of JPX-9 cellular extracts. (E): Western blot analyses were performed with 70 µg of cellular extracts obtained from non-infected (CEM, Jurkat & MOLT4) or HTLV-1-infected (C8166, C91/PL, Hut102 & MT2) T-lymphocytes. (B, C, D, E): Membranes were probed with anti-Gem (1∶2,000) & anti-GAPDH (1∶1,000) or anti-Tax Tab 172 (1∶500) antibodies. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody [NB300-322] - Gem is overexpressed in T- & non-T-Tax-expressing cells as well as in HTLV-1 infected cells.(A): Total RNA was extracted from 293T cells transduced with Lenti-control (lane 2) or Lenti-Tax (lane 3) & RT-PCR was performed using gem or GADPH specific primers. Lane 1 is a control of extraction. (B, C): Western blot analyses were performed with 70 µg of proteins from (B) 293T or (C) MOLT4 cells transduced by Lenti-control or Lenti-Tax vectors (72 h post-transduction). (D): JPX-9 cells were grown for 24 h or 48 h in the presence of ZnCl2 (120 µM). Western blot analyses were performed with 70 µg of JPX-9 cellular extracts. (E): Western blot analyses were performed with 70 µg of cellular extracts obtained from non-infected (CEM, Jurkat & MOLT4) or HTLV-1-infected (C8166, C91/PL, Hut102 & MT2) T-lymphocytes. (B, C, D, E): Membranes were probed with anti-Gem (1∶2,000) & anti-GAPDH (1∶1,000) or anti-Tax Tab 172 (1∶500) antibodies. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-310202416205150.jpg "Western Blot: GAPDH Antibody [NB300-322] - Gem is overexpressed in T- & non-T-Tax-expressing cells as well as in HTLV-1 infected cells.(A): Total RNA was extracted from 293T cells transduced with Lenti-control (lane 2) or Lenti-Tax (lane 3) & RT-PCR was performed using gem or GADPH specific primers. Lane 1 is a control of extraction. (B, C): Western blot analyses were performed with 70 µg of proteins from (B) 293T or (C) MOLT4 cells transduced by Lenti-control or Lenti-Tax vectors (72 h post-transduction). (D): JPX-9 cells were grown for 24 h or 48 h in the presence of ZnCl2 (120 µM). Western blot analyses were performed with 70 µg of JPX-9 cellular extracts. (E): Western blot analyses were performed with 70 µg of cellular extracts obtained from non-infected (CEM, Jurkat & MOLT4) or HTLV-1-infected (C8166, C91/PL, Hut102 & MT2) T-lymphocytes. (B, C, D, E): Membranes were probed with anti-Gem (1∶2,000) & anti-GAPDH (1∶1,000) or anti-Tax Tab 172 (1∶500) antibodies. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
