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G-CSF Antibody (7E4F7) - BSA Free

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Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)
Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)
Immunocytochemistry/ Immunofluorescence: G-CSF Antibody (7E4F7) [NBP2-52447] - Immunofluorescence analysis of A549 cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis of paraffin-embedded esophageal cancer tissues using CSF3 mouse mAb with DAB staining.
Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against HEK293 (1) and CSF3 (AA: 1-207)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis of Hela cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ELISA: G-CSF Antibody (7E4F7) [NBP2-52447] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, CyTOF-ready
Clone
7E4F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52447-0.025mg
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G-CSF Antibody (7E4F7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human G-CSF (AA: 1-207) expressed in E. Coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CSF3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
22.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for G-CSF Antibody (7E4F7) - BSA Free

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3
  • CSF3OS
  • Filgrastim
  • GCSF
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • Pluripoietin

Background

Granulocyte Colony-Stimulating Factor (G-CSF , filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is a 18.7 kDa glycoprotein containing 174 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol CSF3