![Immunohistochemistry-Paraffin: FSH beta Antibody (FSHb/2033R) [NBP3-07794] - Formalin-fixed, paraffin-embedded human pituitary tissue stained with FSH beta Recombinant Rabbit Monoclonal Antibody (FSHb/2033R).](http://images.novusbio.com/fullsize/FSH-beta-Antibody-FSHb-2033R-Immunohistochemistry-Paraffin-NBP3-07794-img0001.jpg "Immunohistochemistry-Paraffin: FSH beta Antibody (FSHb/2033R) [NBP3-07794] - Formalin-fixed, paraffin-embedded human pituitary tissue stained with FSH beta Recombinant Rabbit Monoclonal Antibody (FSHb/2033R).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | FSHb/2033R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08577) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant FSH beta beta sub-unit (Uniprot: P01225) |
| Localization | Cytoplasmic, secreted |
| Specificity | This monoclonal antibody reacts with a protein of 22kDa, identified as beta sub-unit of FSH. It does not cross react with the alpha sub-unit. Follicle stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotrophs in the anterior pituitary gland. In the ovary, FSH stimulates the growth of immature Graafian follicles to maturation. As the follicle grows, it releases inhibin, which deactivates the FSH production. In men, FSH enhances the production of androgen-binding protein by the Sertoli cells of the testis and is critical for spermatogenesis. FSH and LH act synergistically in reproduction. FSH is a useful marker in the classification of pituitary tumors and the study of pituitary disease. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | FSHB |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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