![Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mouse mAb against A549 (1), and PC-3 (2) cell lysate.](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Western-Blot-NBP2-37451-img0003.jpg "Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mouse mAb against A549 (1), and PC-3 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, CyTOF-ready |
| Clone | 2D12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Frizzled-5 (AA:151-217) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FZD5 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 64.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Frizzled-5 Antibody (NBP2-37451)Find related products by research area.
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| Gene Symbol | FZD5 |
![Flow Cytometry: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis of HepG2 cells using FZD5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Flow-Cytometry-NBP2-37451-img0004.jpg "Flow Cytometry: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis of HepG2 cells using FZD5 mouse mAb (green) and negative control (red).")
![Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mAb against human FZD5 recombinant protein. (Expected MW is 32.5 kDa).](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Western-Blot-NBP2-37451-img0002.jpg "Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mAb against human FZD5 recombinant protein. (Expected MW is 32.5 kDa).")
![ELISA: Frizzled-5 Antibody (2D12) [NBP2-37451] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-ELISA-NBP2-37451-img0001.jpg "ELISA: Frizzled-5 Antibody (2D12) [NBP2-37451] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")