| Reactivity | HuSpecies Glossary |
| Concentration | Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance. |
| Application Notes | The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are nonmurine in origin, hence avoiding the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells). Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture. Amplify the plasmid DNA by transforming on to a suitable competent bacteria, e.g. DH5alpha or XL-1 Blue. Plate on LB agar plate containing 50ug/ml ampicillin. Start a culture from a single colony for plasmid DNA preparation. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA) |
| Concentration | Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance. |
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Research Areas for FoxP3 (NBP2-29545)Find related products by research area.
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Meeting Report: 2nd International Antibody Validation Meeting Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde... Read full blog post. |
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FOXP3 Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function. Distinctively, ... Read full blog post. |
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FOXP3 is a Master Regulator of T Regulatory (Treg) Cells FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ... Read full blog post. |
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FOXP3: Master Regulatory Transcriptional Factor FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom... Read full blog post. |
| Gene Symbol | FOXP3 |
![FoxP3 [NBP2-29545] - FOXP3 [NBP2-29545] - Schematic representation](http://images.novusbio.com/fullsize/FoxP3-NBP2-29545-img0002.jpg "FoxP3 [NBP2-29545] - FOXP3 [NBP2-29545] - Schematic representation")
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![Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](https://images.novusbio.com/images/FoxP3-Antibody-Flow-Cytometry-NB100-39002-img0021.jpg "Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")