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FoxP3 Antibody [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, Dual ISH-IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-245AF350
Alexa Fluor 405
NB600-245AF405
Alexa Fluor 488
NB600-245AF488
Alexa Fluor 532
NB600-245AF532
Alexa Fluor 594
NB600-245AF594
Alexa Fluor 647
NB600-245AF647
Alexa Fluor 700
NB600-245AF700
Alexa Fluor 750
NB600-245AF750
Allophycocyanin
NB600-245APC
Biotin
NB600-245B
CoraFluor 1
NB600-245CL1
DyLight 350
NB600-245UV
DyLight 405
NB600-245V
DyLight 488
NB600-245G
DyLight 550
NB600-245R
DyLight 594
NB600-245DL594
DyLight 650
NB600-245C
DyLight 680
NB600-245FR
DyLight 755
NB600-245IR
FITC
NB600-245F
HRP
NB600-245H
Janelia Fluor 525
NB600-245JF525
Janelia Fluor 549
NB600-245JF549
Janelia Fluor 585
NB600-245JF585
Janelia Fluor 635
NB600-245JF635
Janelia Fluor 646
NB600-245JF646
PE
NB600-245PE
PerCP
NB600-245PCP
Unconjugated
NB600-245
mFluor Violet 450 SE
NB600-245MFV450
mFluor Violet 500 SE
NB600-245MFV500
mFluor Violet 610 SE
NB600-245MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB600-245
BSA Free
NB600-245
BSA Free
NB600-245SS
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FoxP3 Antibody [Janelia Fluor® 669] Summary

Immunogen
A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431). [UniProt# Q9BZS1]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXP3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

This antibody does not appear to detect the mouse FOXP3 protein by western blot, but does work on mouse tissue for IHC.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for FoxP3 Antibody [Janelia Fluor® 669]

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

Forkhead box P3 (FoxP3), aka Scurfin and JM2, is a protein involving in immune system responses and functioning as a transcriptional regulator crucial in the development and inhibitory of regulatory T-Cells (Treg). FoxP3 is a master regulator of the Treg lineage, who requires Ahr and STAT-5 and other additional transcription factors to retains its complete function and differentiation. Scurfin remains steady and constitutively expressed in two conditions: a high level in CD25 + CD4 positive regulatory T cells and a low level in CD4 positive/CD25 negative cells. JM2 is absent in CD4 negative/CD8 positive T cells. FoxP3 defects are related to various cancers and autoimmune diseases, and X-linked syndrome (IPEX), while overexpression may also cause severe immunodeficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxP3 Antibody (NB600-245JF669). (Showing 1 - 2 of 2 FAQ).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Secondary Antibodies

 

Isotype Controls

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Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.
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Bioinformatics

Gene Symbol FOXP3