![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - (5ug/ml) staining of paraffin embedded Mouse Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NB600-243_Immunohistochemistry-Paraffin-FoxP3-Antibody-NB600-243-5ug-ml-staining-of-paraffin-embedded-Mouse-Spleen-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254016.jpg "Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - (5ug/ml) staining of paraffin embedded Mouse Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.")
| Reactivity | MuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence KRSQRPNKCSNP corresponding to C-Terminus according to Uniprot Mouse NP_473380.1. |
| Localization | Nuclear |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | FOXP3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P: Mouse thymus shows nuclear staining in some patches of thymocytes. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![Flow Cytometry GITR/TNFRSF18 Antibody (110416) [Unconjugated]](https://images.novusbio.com/images/antibody/mab689_human-gitr-tnfrsf18-mab-clone-110416-flow-cytometry-286202414437..jpg)
| Publication using NB600-243 | Applications | Species |
|---|---|---|
| Brunkow,M.E, Jeffery et al. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat Genet 2001-01-01 [PMID: 11138001] |
Secondary Antibodies |
Isotype Controls |
Research Areas for FoxP3 Antibody (NB600-243)Find related products by research area.
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Meeting Report: 2nd International Antibody Validation Meeting Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde... Read full blog post. |
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FOXP3 Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function. Distinctively, ... Read full blog post. |
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FOXP3 is a Master Regulator of T Regulatory (Treg) Cells FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ... Read full blog post. |
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FOXP3: Master Regulatory Transcriptional Factor FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FOXP3 |
| Entrez | |
| Uniprot |
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![Flow Cytometry: FoxP3 Antibody [NB600-243] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Flow-Cytometry-NB600-243-img0003.jpg "Flow Cytometry: FoxP3 Antibody [NB600-243] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - Negative Control showing staining of paraffin embedded Mouse Spleen, with no primary antibody.](http://images.novusbio.com/fullsize/NB600-243_Immunohistochemistry-Paraffin-FoxP3-Antibody-NB600-243-Negative-Control-showing-staining-of-paraffin-embedded-Mouse-Spleen-with-no-primary-antibody--29320239254029.jpg "Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - Negative Control showing staining of paraffin embedded Mouse Spleen, with no primary antibody.")
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