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FoxP3 Antibody (1054C) [Allophycocyanin]

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Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse Anti-Human IL‑2 R alpha (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB1020P), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 APC‑conjugated Monoclonal Antibody (Catalog # IC8214A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.
Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse ...read more
C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and (B) Rat Anti-Mouse IL‑2 R alpha (CD25) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
1054C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC8214U-100UG
Alexa Fluor 405
IC8214V-100UG
Alexa Fluor 488
IC8214G-100
Alexa Fluor 532
FAB8214X
Alexa Fluor 594
IC8214T-100UG
Alexa Fluor 647
IC8214R-100UG
Alexa Fluor 700
IC8214N-100
Alexa Fluor 750
IC8214S-100UG
Allophycocyanin
FPK8214A-100
Allophycocyanin
FPK8214A-025
Allophycocyanin/Cy7
FAB8214APCCY7
Biotin
FAB8214B
CoraFluor 1
FAB8214CL1
DyLight 350
FAB8214D
DyLight 405
FAB8214E
DyLight 488
FAB8214K
DyLight 550
FAB8214L
DyLight 594
FAB8214M
DyLight 650
FAB8214W
DyLight 680
FAB8214Y
DyLight 755
FAB8214Z
FITC
FAB8214F
HRP
FAB8214H
Janelia Fluor 525
FAB8214JF525
Janelia Fluor 549
FAB8214I
Janelia Fluor 585
FAB8214JF585
Janelia Fluor 635
FAB8214JF635
Janelia Fluor 646
FAB8214J
Janelia Fluor 669
FAB8214JF669
PE/Atto594
FAB8214PEATT594
PE/Cy5.5
FAB8214PECY55
PE/Cy7
FAB8214PECY7
PerCP
FAB8214C
Phycoerythrin
FPK8214P-100
Phycoerythrin
FPK8214P-025
Phycoerythrin
IC8214P-100
Unconjugated
MAB8214
mFluor Violet 450 SE
FAB8214MFV450
mFluor Violet 500 SE
FAB8214MFV500
mFluor Violet 610 SE
FAB8214MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB8214
Carrier Free
IC8214P-100
Carrier Free
IC8214G-100
Carrier Free
IC8214N-100
Carrier Free
FPK8214A-025
Carrier Free
FPK8214A-100
Carrier Free
FPK8214P-025
Carrier Free
FPK8214P-100
Carrier Free
MAB8214
Carrier Free
MAB8214-SP
Carrier Free
IC8214V-100UG
Carrier Free
IC8214U-100UG
Carrier Free
IC8214T-100UG
Carrier Free
IC8214S-100UG
Carrier Free
IC8214R-100UG
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FoxP3 Antibody (1054C) [Allophycocyanin] Summary

Additional Information
Recombinant Monoclonal Antibody. Use with Catalog # FC012, FlowX FoxP3 Fixation & Permeabilization Buffer Kit
Immunogen
E. coli-derived recombinant human FoxP3
Met1-Leu71
Accession # Q9BZS1
Specificity
Detects human and mouse FoxP3 in flow cytometry.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FOXP3
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
IC8214A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxP3 Antibody (1054C) [Allophycocyanin]

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxP3 Antibody (IC8214A)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(1)
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Reviews for FoxP3 Antibody (IC8214A) (0)

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FAQs for FoxP3 Antibody (IC8214A). (Showing 1 - 3 of 3 FAQ).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the immunogen sequence of this FoxP3 antibody?
    • A sequence from Met1 - Leu71.

Secondary Antibodies

 

Isotype Controls

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Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.
FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
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Bioinformatics

Gene Symbol FOXP3
Uniprot