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FoxO1/FKHR Antibody [Unconjugated]

 
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Western blot shows lysates of 293T human embryonic kidney cell line and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human FoxO1/FKHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5939) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxO1/FKHR at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of 293T human embryonic kidney cell line and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human FoxO1/FKHR Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of 293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxO1/FKHR at approximately 93 kDa (as indicated) using 10 µg/mL of Sheep ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF5939-SP
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FoxO1/FKHR Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FoxO1/FKHR
Ala353-Gly655
Accession # Q12778
Specificity
Detects human FoxO1/FKHR in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
FOXO1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxO1/FKHR Antibody [Unconjugated]

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

FoxO1, also called FKHR (forkhead in ­rhabdomyosarcoma), is a 655 amino acid (aa), 70 kDa, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity. A 60 kDa form, cleaved at R537, has been found in androgen-treated prostate cancer cells. Over aa 353‑655, human FoxO1/FKHR shares 93% aa identity with mouse and rat FoxO1/FKHR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FOXO1
Uniprot