Name: p27/Kip1 [p Thr198] Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF3994
Price: 399 USD

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	LYOPH

Immunogen	Phosphopeptide containing human p27/Kip1 T198 site
Modification	p Thr198
Specificity	Detects human p27/Kip1 when phosphorylated at T198. Does not recognize p27/Kip1 when unphosphorylated at T198.
Details of Functionality	None<br /><br />
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	CDKN1B
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Publications using AF3994	Applications	Species
A Nowosad, P Jeannot, C Callot, J Creff, RT Perchey, C Joffre, P Codogno, S Manenti, A Besson p27 controls Ragulator and mTOR activity in amino acid-deprived cells to regulate the autophagy-lysosomal pathway and coordinate cell cycle and cell growth Nat. Cell Biol., 2020-08-17;22(9):1076-1090. 2020-08-17 [PMID: 32807902] (Western Blot, Human)	Western Blot	Human
YF Lian, YL Huang, YJ Zhang, DM Chen, JL Wang, H Wei, YH Bi, ZW Jiang, P Li, MS Chen, YH Huang CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation Theranostics, 2019-10-22;9(26):8392-8408. 2019-10-22 [PMID: 31754404] (Western Blot, Human)	Western Blot	Human
CF Theriau, MK Connor Voluntary physical activity counteracts the proliferative tumor growth microenvironment created by adipose tissue via high-fat diet feeding in female rats Physiol Rep, 2017-07-01;5(13):. 2017-07-01 [PMID: 28676553] (Western Blot, Human)	Western Blot	Human
Dai Horiuchi, Roman Camarda, Alicia Y. Zhou, Christina Yau, Olga Momcilovic, Sanjeev Balakrishnan et al. PIM kinase inhibition presents a novel targeted therapy against triple-negative breast tumors with elevated MYC expression Nature Medicine 11/1/2016 [PMID: 27775705]
Na Zhou, Shoudao Yuan, Rongchun Wang, Weifang Zhang, Jason J. Chen Role of dual specificity tyrosine-phosphorylation-regulated kinase 1B (Dyrk1B) in S-phase entry of HPV E7 expressing cells from quiescence Oncotarget 8/19/2015 [PMID: 26307683]
Hogel H, Miikkulainen P, Bino L, Jaakkola PM. Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27. Mol. Cancer. 7/30/2015 [PMID: 26223520] Details: PHD1 antibody used for WB on 786-O cell under hypoxia (1% O2) and normoxia (21 % O2) by siPHD1 (Figure 1C).
Carmela De Marco, Donatella Malanga, Nicola Rinaldo, Fernanda De Vita, Marianna Scrima, Sara Lovisa et al. Mutant AKT1-E17K is oncogenic in lung epithelial cells Oncotarget 5/25/2015 [PMID: 26053093]
Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation. Oncogene, 2015-02-16;34(43):5447-59. 2015-02-16 [PMID: 25684140] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human
Yanagi T, Krajewska M, Matsuzawa SI et al. PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells. Cancer Res 10/15/2014 [PMID: 25205104]
Moros A, Bustany S, Cahu J, Saborit-Villarroya I, Martinez A, Colomer D, Sola B, Roue G Antitumoral activity of lenalidomide in in vitro and in vivo models of mantle cell lymphoma involves the destabilization of cyclin D1/p27KIP1 complexes. Clin Cancer Res, 2013-10-31;20(2):393-403. 2013-10-31 [PMID: 24178620] (ICC, Immunoprecipitation, Western Blot, Human)	ICC, Immunoprecipitation, Western Blot	Human
Lin , Victor T, Lin , Vivian Y, Lai , Yun-Ju, Chen , Chen-Sha, Liu , Kang, Lin , Weei-Chi, Lin , Fang-Tsy TRIP6 regulates p27 KIP1 to promote tumorigenesis. Mol Cell Biol, 2013-01-22;33(7):1394-409. 2013-01-22 [PMID: 23339869] (Western Blot, Human)	Western Blot	Human
Dumitru CA, Fechner MK, Hoffmann TK A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer. J. Leukoc. Biol., 2012-01-18;91(4):591-8. 2012-01-18 [PMID: 22262799] (Western Blot, Human)	Western Blot	Human
Uranbileg B, Yamamoto H, Park JH Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation. J. Biol. Chem., 2012-01-05;287(9):6275-83. 2012-01-05 [PMID: 22223646] (Western Blot, Rat)	Western Blot	Rat
Wen S, So Y, Singh K Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer. Oncogene, 2011-08-15;31(14):1771-80. 2011-08-15 [PMID: 21841827] (Western Blot, Human)	Western Blot	Human
Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility. J. Biol. Chem., 2011-06-29;286(33):29005-13. 2011-06-29 [PMID: 21715331] (Western Blot, Human)	Western Blot	Human
Donohoe DR, Garge N, Zhang X, Sun W, O'Connell TM, Bunger MK, Bultman SJ The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon. Cell Metab., 2011-05-04;13(5):517-26. 2011-05-04 [PMID: 21531334] (Western Blot, Mouse)	Western Blot	Mouse
Santi SA, Lee H Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells. PLoS ONE, 2011-01-31;6(1):e14614. 2011-01-31 [PMID: 21297943] (Western Blot, Human)	Western Blot	Human
Bustany S, Tchakarska G, Sola B Cyclin D1 regulates p27(Kip1) stability in B cells. Cell. Signal., 2010-09-15;23(1):171-9. 2010-09-15 [PMID: 20837141] (Western Blot, Mouse)	Western Blot	Mouse
Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma. Clin. Cancer Res., 2009-01-01;15(1):81-90. 2009-01-01 [PMID: 19118035] (Western Blot, Human)	Western Blot	Human
Show All 19 Publications. Collapse Publications.

p27/Kip1 [p Thr198] Antibody [Unconjugated]

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Carrier Free AF3994-SP