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FOXI1 Antibody (PCRP-FOXL1-1F8)

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Immunocytochemistry/ Immunofluorescence: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Immunofluorescence analysis of PFA-fixed HeLa cells. FOXI1 Antibody (PCRP-FOXL1-1F8) followed by goat anti-mouse IgG-CF488 (green). ...read more
Immunohistochemistry-Paraffin: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Formalin-fixed, paraffin-embedded human prostate stained. FOXI1 antibody (PCRP-FOXL1-1F8) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. ...read more
Flow Cytometry: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Flow cytometric analysis of PFA-fixed HeLa cells. FOXI1 antibody (PCRP-FOXL1-1F8) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Protein Array: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Analysis of Protein Array containing more than 19,000 full-length human proteins using FOXI1 Antibody (PCRP-FOXL1-1F8).
Protein Array: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Analysis of Protein Array containing more than 19,000 full-length human proteins using FOXI1 Antibody (PCRP-FOXL1-1F8).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, MA
Clone
PCRP-FOXL1-1F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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FOXI1 Antibody (PCRP-FOXL1-1F8) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14224)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.

Theoretical molecular weight: 36-41kDa
Immunogen
Recombinant fragment (around aa104-258) of human FOXI1 protein (exact sequence is proprietary) (Uniprot: Q12951)
Localization
Nucleus
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOXI1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Reactivity Notes

Predicted to react in Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for FOXI1 Antibody (PCRP-FOXL1-1F8)

  • FKHL10HFH3
  • forkhead box I1
  • forkhead box protein I1
  • forkhead-like 10
  • forkhead-related activator 6
  • Forkhead-related protein FKHL10
  • Forkhead-related transcription factor 6
  • FREAC-6
  • FREAC6FKH10
  • Hepatocyte nuclear factor 3 forkhead homolog 3
  • HFH-3
  • HNF-3/fork-head homolog 3
  • HNF-3/fork-head homolog-3
  • MGC34197

Background

FOXI1 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FOXI1
Uniprot