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FOXI1 Antibody (OTI1D4) - Azide and BSA Free

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Western Blot: FOXI1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-70747] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1.
Western Blot: FOXI1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-70747] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: FOXI1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-70747] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1.
Immunohistochemistry: FOXI1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-70747] - Staining of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.
Flow Cytometry: FOXI1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-70747] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FOXI1 ...read more
Immunohistochemistry: FOXI1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-70747] - Staining of paraffin-embedded Human prostate tissue using anti-FOXI1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70747AF350
Alexa Fluor 405
NBP2-70747AF405
Alexa Fluor 488
NBP2-70747AF488
Alexa Fluor 532
NBP2-70747AF532
Alexa Fluor 594
NBP2-70747AF594
Alexa Fluor 647
NBP2-70747AF647
Alexa Fluor 700
NBP2-70747AF700
Alexa Fluor 750
NBP2-70747AF750
Allophycocyanin
NBP2-70747APC
Allophycocyanin/Cy7
NBP2-70747APCCY7
Biotin
NBP2-70747B
CoraFluor 1
NBP2-70747CL1
DyLight 350
NBP2-70747UV
DyLight 405
NBP2-70747V
DyLight 488
NBP2-70747G
DyLight 550
NBP2-70747R
DyLight 594
NBP2-70747DL594
DyLight 650
NBP2-70747C
DyLight 680
NBP2-70747FR
DyLight 755
NBP2-70747IR
FITC
NBP2-70747F
HRP
NBP2-70747H
Janelia Fluor 525
NBP2-70747JF525
Janelia Fluor 549
NBP2-70747JF549
Janelia Fluor 585
NBP2-70747JF585
Janelia Fluor 635
NBP2-70747JF635
Janelia Fluor 646
NBP2-70747JF646
Janelia Fluor 669
NBP2-70747JF669
PE
NBP2-70747PE
PE/Atto594
NBP2-70747PEATT594
PE/Cy5.5
NBP2-70747PECY55
PE/Cy7
NBP2-70747PECY7
PerCP
NBP2-70747PCP
Unconjugated
NBP2-03868
mFluor Violet 450 SE
NBP2-70747MFV450
mFluor Violet 500 SE
NBP2-70747MFV500
mFluor Violet 610 SE
NBP2-70747MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-03868
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FOXI1 Antibody (OTI1D4) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 111-350 of human FOXI1 (NP_036320) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOXI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
40.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for FOXI1 Antibody (OTI1D4) - Azide and BSA Free

  • FKHL10HFH3
  • forkhead box I1
  • forkhead box protein I1
  • forkhead-like 10
  • forkhead-related activator 6
  • Forkhead-related protein FKHL10
  • Forkhead-related transcription factor 6
  • FREAC-6
  • FREAC6FKH10
  • Hepatocyte nuclear factor 3 forkhead homolog 3
  • HFH-3
  • HNF-3/fork-head homolog 3
  • HNF-3/fork-head homolog-3
  • MGC34197

Background

FOXI1 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FOXI1