![Western Blot: FMRP [p Ser499] Antibody [NBP2-29509] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71k FMRP protein phosphorylated at Ser499 (Control). The phosphospecificity of this labeling is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min) before being exposed to the FMRP Ser499 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/FMRP-[p-Ser499]-Antibody-Western-Blot-NBP2-29509-img0002.jpg "Western Blot: FMRP [p Ser499] Antibody [NBP2-29509] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71k FMRP protein phosphorylated at Ser499 (Control). The phosphospecificity of this labeling is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min) before being exposed to the FMRP Ser499 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
| Reactivity | Hu, Mu, Rt, Ch, Hu, Bv, Ca, Ch, Pm, Sh, Xp, ZeSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 conjugated to KLH. Accession # Q80WE1 |
| Modification | p Ser499 |
| Specificity | Specific for endogenous levels of the ~71 kDa FMRP protein phosphorylated at Ser499. Immunolabeling is completely eliminated by treatment with lambda-phosphatase. |
| Preparation Method |
Prepared from rabbit serum by affinity purification via sequentia chromatography on phospho- and dephosphopeptide affinity columns. |
| Predicted Species | Human (100%), Sheep (100%), Canine (100%), Chicken (100%), Xenopus (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FMR1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 71 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for FMRP Antibody (NBP2-29509)Find related products by research area.
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