FLIP Antibody - BSA Free

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Showing Publications 1 - 9 of 9.

Publications using NBP1-77020	Applications	Species
Kim JH, Gupta SC, Park B et al. Turmeric (Curcuma longa) inhibits inflammatory nuclear factor (NF)-kB and NF-kB-regulated gene products and induces death receptors leading to suppressed proliferation, induced chemosensitization, and suppressed osteoclastogenesis. Mol Nutr Food Res. 2012-03-01 [PMID: 22147524] Details: 1. SEAPorter Assay kit (10055k): Fig 2B, Analysis of the effects of tumeric on the inhibition of TNF-induced NF-kB activation in NF-kB SEAP stably transfected cells. 2. FLIP/I-FLiCE pAb (IMG-116-1/116-2): Fig 5C (WB), HCT-116 cells treated with tumeric.
Phromnoi K, Reuter S, Sung B et al. A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IkB kinase activation and sensitizes tumor cells to apoptosis by cytokines and chemotherapeutic agents. Mol Pharmacol. 2011-02-01 [PMID: 20930110] Details: KBM-5 cells: 1. FLIP (IMG-116-1, -2): WB (Fig 4A) 2. IKKb/IKK2 (IMG-129A): IP Kinase Assay (Fig 2D), IP/WB (Fig 2D). Note, the antibody IP'd an active kinase. The antibody co'IP'd IKKa. 3. IKKa (IMG-136a): WB (Figs 2C, 2D). Note Fig 2D was generated with an IP (IKKb, IMG-129A)/WB (IKKa, IMG-136a) assay. Note: The KBM-5 (human chronic myeloid leukemia) cells were incubated with PMF then treated with TNF-alpha, Figs 2C, 2D, 4A.
Aggarwal S, Ichikawa H, Takada Y et al. Curcumin (diferuloylmethane) down-regulates expression of cell proliferation and antiapoptotic and metastatic gene products through suppression of IkappaBalpha kinase and Akt activation. Mol Pharmacol. 2006-01-01 [PMID: 16219905]
Ichikawa H, Takada Y, Murakami A, Aggarwal BB. Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products. J Immunol. 2005-06-01 [PMID: 15905586]
Takada Y, Andreeff M, Aggarwal BB. Indole-3-carbinol suppresses NF-kappaB and IkappaBalpha kinase activation, causing inhibition of expression of NF-kappaB-regulated antiapoptotic and metastatic gene products and enhancement of apoptosis in myeloid and leukemia cells. Blood. 2005-07-15 [PMID: 15811958]
Takada Y, Fang X, Jamaluddin MS et al. Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor. J Biol Chem. 2004-09-17 [PMID: 15252041]
Takada Y, Khuri FR, Aggarwal BB. Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis. J Biol Chem. 2004-06-18 [PMID: 15090542]
Kishimoto H, Sprent J. A defect in central tolerance in NOD mice. Nat Immunol. 2001-11-01 [PMID: 11668341]
Kim K, Fisher MJ, Xu SQ, el-Deiry WS. Molecular determinants of response to TRAIL in killing of normal and cancer cells. Clin Cancer Res. 2000-02-01 [PMID: 10690508]

Array NBP1-77020

• FLIP Antibodies
• FLIP ELISA Kits
• FLIP Lysates
• FLIP Proteins
• FLIP Proteins and Enzyme