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Fibulin-3/EFEMP1 Antibody - BSA Free

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Western Blot: Fibulin-3/EFEMP1 Antibody [NBP1-77040] - HeLa cell lysate with EFEMP1 antibody at (A) 0.5 and (B) 1 ug/ml.
Western Blot: Fibulin-3/EFEMP1 Antibody [NBP1-77040] - HeLa cell lysate with EFEMP1 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP1-77040] - Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP1-77040] - HeLa cells with EFEMP1 antibody at 20 ug/ml.
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody - BSA Free [NBP1-77040] - Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 u/mL.
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody - BSA Free [NBP1-77040] - Immunocytochemistry of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 u/mL.
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody - BSA Free [NBP1-77040] - Immunocytochemistry of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 u/mL.
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody - BSA Free [NBP1-77040] - Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 u/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-77040AF350
Alexa Fluor 405
NBP1-77040AF405
Alexa Fluor 488
NBP1-77040AF488
Alexa Fluor 532
NBP1-77040AF532
Alexa Fluor 594
NBP1-77040AF594
Alexa Fluor 647
NBP1-77040AF647
Alexa Fluor 700
NBP1-77040AF700
Alexa Fluor 750
NBP1-77040AF750
Biotin
NBP1-77040B
CoraFluor 1
NBP1-77040CL1
DyLight 350
NBP1-77040UV
DyLight 405
NBP1-77040V
DyLight 488
NBP1-77040G
DyLight 550
NBP1-77040R
DyLight 594
NBP1-77040DL594
DyLight 650
NBP1-77040C
DyLight 680
NBP1-77040FR
DyLight 755
NBP1-77040IR
FITC
NBP1-77040F
HRP
NBP1-77040H
Janelia Fluor 525
NBP1-77040JF525
Janelia Fluor 549
NBP1-77040JF549
Janelia Fluor 585
NBP1-77040JF585
Janelia Fluor 635
NBP1-77040JF635
Janelia Fluor 646
NBP1-77040JF646
Janelia Fluor 669
NBP1-77040JF669
PerCP
NBP1-77040PCP
mFluor Violet 450 SE
NBP1-77040MFV450
mFluor Violet 500 SE
NBP1-77040MFV500
mFluor Violet 610 SE
NBP1-77040MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-77040-0.025mg
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Fibulin-3/EFEMP1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3. The immunogen is located within amino acids 410 - 460 of Fibulin 3. Amino Acid Squence: TFRIKSGNENGEFYLRQTS
Specificity
At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFEMP1
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 0.5-1 ug/ml
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Fibulin-3/EFEMP1 Peptide (NBP1-77040PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Fibulin-3/EFEMP1 Antibody - BSA Free

  • DHRD
  • EFEMP1
  • EGF containing fibulin-like extracellular matrix protein 1
  • EGF-containing fibulin-like extracellular matrix protein 1
  • Extracellular protein S1-5
  • FBLN3
  • FBLN3DRAD
  • FBNLFLJ35535
  • FIBL-3
  • Fibrillin-like protein
  • fibrillin-like
  • Fibulin 3
  • fibulin-3
  • MGC111353
  • MLVT
  • MTLV
  • S1-5

Background

Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target. At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EFEMP1
Entrez
Uniprot