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Fgr Antibody (3G10)

Images

 
Western Blot: Fgr Antibody (3G10) [H00002268-M01] - FGR monoclonal antibody (M01), clone 3G10. Analysis of FGR expression in Raw 264.7.
Immunohistochemistry-Paraffin: Fgr Antibody (3G10) [H00002268-M01] - Analysis of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
Western Blot: Fgr Antibody (3G10) [H00002268-M01] - FGR monoclonal antibody (M01), clone 3G10 Analysis of FGR expression in HeLa.
Western Blot: Fgr Antibody (3G10) [H00002268-M01] - FGR monoclonal antibody (M01), clone 3G10. Analysis of FGR expression in PC-12.
Sandwich ELISA: Fgr Antibody (3G10) [H00002268-M01] - Detection limit for recombinant GST tagged FGR is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
3G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Fgr Antibody (3G10) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
FGR (AAH64382, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCVFCKKLEPVATAKEDAGLEGDFRSYGAADHYGPDPTKARPASSFAHIPNYSNFSSQAINPGFLDSGTIRGVSGIGVTLFIALYDYEA
Specificity
FGR - Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FGR
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Fgr Antibody (3G10)

  • c-fgr
  • c-src-2 proto-oncogene
  • c-src2
  • EC 2.7.10
  • EC 2.7.10.2
  • Fgr
  • FLJ43153
  • Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
  • MGC75096
  • p55-c-fgr protein
  • p55c-fgr
  • p55-Fgr
  • p58c-fgr
  • Proto-oncogene c-Fgr
  • proto-oncogene tyrosine-protein kinase FGR
  • Src2
  • SRC2c-fgr protooncogene
  • tyrosine-protein kinase Fgr

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FGR
Entrez
OMIM
Uniprot