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Recombinant Human FGF acidic/FGF1 Protein

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SDS-Page: Recombinant Human FGF acidic/FGF1 Protein [NBP2-61310]
SDS-Page: Recombinant Human FGF acidic/FGF1 Protein [NBP2-61310]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Bioactivity
Concentration
LYOPH

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Recombinant Human FGF acidic/FGF1 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 154 amino acids corresponding to FGF acidic/FGF1 Source: E. coli

Uniprot ID: P05230

Amino Acid Sequence: AEGEITTFTA LTEKFNLPPG NYKKPKLLYC SNGGHFLRIL PDGTVDGTRD RSDQHIQLQL SAESVGEVYI KSTETGQYLA MDTDGLLYGS QTPNEECLFL ERLEENHYNT YISKKHAEKN WFVGLKKNGS CKRGPRTHYG QKAILFLPLP VSSD
Details of Functionality
FGF acidic/FGF1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg in the presence of 10 ug/ml of heparin.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
FGF1
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of FGF acidic/FGF1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Western Blot
Theoretical MW
17.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 2 mM EDTA, 0.5 mM DTT.
Preservative
5% Trehalose
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human FGF acidic/FGF1 Protein

  • AFGF
  • alpha
  • alpha-ECGF
  • beta-ECGF
  • ECGF
  • ECGFB
  • ECGF-betaAcidic fibroblast growth factor
  • endothelial cell growth factor, beta
  • FGF acidic
  • FGF-1
  • FGFABeta-endothelial cell growth factor
  • FGF-alpha
  • fibroblast growth factor 1 (acidic)
  • GLIO703
  • HBGF1
  • HBGF-1
  • heparin-binding growth factor 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol FGF1