Home » FGF acidic/FGF1 » FGF acidic/FGF1 Lysates » FGF acidic/FGF1 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

FGF acidic/FGF1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: FGF1 Overexpression Lysate (Adult Normal) [NBP2-09719] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGF1.
Western Blot: FGF1 Overexpression Lysate (Adult Normal) [NBP2-09719] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGF1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

FGF acidic/FGF1 Overexpression Lysate Summary

Description

FGF acidic/FGF1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC227797

Accession#: NM_001144935

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
FGF1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
17.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for FGF acidic/FGF1 Overexpression Lysate

  • AFGF
  • alpha
  • alpha-ECGF
  • beta-ECGF
  • ECGF
  • ECGFB
  • ECGF-betaAcidic fibroblast growth factor
  • endothelial cell growth factor, beta
  • FGF acidic
  • FGF-1
  • FGFABeta-endothelial cell growth factor
  • FGF-alpha
  • fibroblast growth factor 1 (acidic)
  • GLIO703
  • HBGF1
  • HBGF-1
  • heparin-binding growth factor 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(550 Publications)
Species: Hu
Applications: BA
FGFR1 Antibody
(M19B2) - BSA Free
NB600-1287
Flow Cytometry FGFR1 Antibody (M19B2) - BSA Free
Flow Cytometry FGFR1 Antibody (M19B2) - BSA Free
Western Blot FGFR1 Antibody (M19B2) - BSA Free
(1 Review)
(7 Publications)
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
FGFR2 Antibody
(1G3)
H00002263-M01
Immunohistochemistry-Paraffin FGFR2 Antibody (1G3)
Western Blot FGFR2 Antibody (1G3)
Sandwich ELISA FGFR2 Antibody (1G3)
(13 Publications)
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(842 Publications)
Species: Hu
Applications: BA
KGF/FGF-7
[Unconjugated]
251-KG
Bioactivity KGF/FGF-7 [Unconjugated]
Bioactivity KGF/FGF-7 [Unconjugated]
(85 Publications)
Species: Hu
Applications: BA
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(684 Publications)
Species: Hu
Applications: ELISA
FGF-13 Antibody
NBP2-16471
Immunohistochemistry-Paraffin FGF-13 Antibody
Western Blot FGF-13 Antibody
Immunohistochemistry-Paraffin FGF-13 Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
FGF-4
[Unconjugated]
235-F4
SDS-PAGE FGF-4 [Unconjugated]
Bioactivity FGF-4 [Unconjugated]
(75 Publications)
Species: Hu
Applications: BA
HGF [Unconjugated]
294-HG
Bioactivity HGF [Unconjugated]
Cell Culture HGF [Unconjugated]
(113 Publications)
Species: Hu
Applications: BA
IGF-I/IGF-1
[Unconjugated]
291-G1
SDS-PAGE IGF-I/IGF-1 [Unconjugated]
Bioactivity IGF-I/IGF-1 [Unconjugated]
Mass Spectrometry IGF-I/IGF-1 [Unconjugated]
(224 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(663 Publications)
Species: Mu
Applications: ELISA
FGF-10
[Unconjugated]
345-FG
SDS-PAGE FGF-10 [Unconjugated]
Bioactivity FGF-10 [Unconjugated]
Bioactivity FGF-10 [Unconjugated]
(103 Publications)
Species: Hu
Applications: BA
FGFR3 Antibody
(136312) [Unconjugate ...
MAB7662
Immunohistochemistry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
Flow Cytometry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
Immunocytochemistry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
(1 Review)
(3 Publications)
Species: Hu
Applications: Flow, ICC, IHC, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
FGFR4 Antibody
(240929) [Unconjugate
MAB6852
Immunocytochemistry FGFR4 Antibody (240929) [Unconjugated]
Immunohistochemistry FGFR4 Antibody (240929) [Unconjugated]
Western Blot FGFR4 Antibody (240929) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(817 Publications)
Species: Hu
Applications: BA
FGF-8
[Unconjugated]
423-F8
SDS-PAGE FGF-8 [Unconjugated]
Bioactivity FGF-8 [Unconjugated]
Cell Culture FGF-8 [Unconjugated]
(1 Review)
(63 Publications)
Species: Hu, Mu
Applications: BA

Publications for FGF acidic/FGF1 Lysate (NBP2-09719) (0)

There are no publications for FGF acidic/FGF1 Lysate (NBP2-09719).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FGF acidic/FGF1 Lysate (NBP2-09719) (0)

There are no reviews for FGF acidic/FGF1 Lysate (NBP2-09719). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FGF acidic/FGF1 Lysate (NBP2-09719) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional FGF acidic/FGF1 Products

Research Areas for FGF acidic/FGF1 Lysate (NBP2-09719)

Find related products by research area.

Blogs on FGF acidic/FGF1

There are no specific blogs for FGF acidic/FGF1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FGF acidic/FGF1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol FGF1