FFAR4/GPR120 Antibody - BSA Free

GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source. They also employ various physiological responses through their GPCRs. One such response occurs when dietary FFAs stimulate GPR120. This stimulation promotes the secretion of Glucagon-like peptide-1 (GLP-1) in vivo and in vitro.GLP-1 belongs to the class of molecules known as the incretins, which are associated with insulin secreted from the pancreas as a result of food intake. GLP-1 also inhibits Glucagon and gastric acid secretion and gastric emptying. Consequently, the role of GPR120 in the secretion of GLP-1 is critical in the treatment of diabetes.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/IF, IF/IHC, IHC-P, WB.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using NBP1-00858	Applications	Species
Karmokar PF, Moniri NH Free-Fatty Acid Receptor-4 (FFA4/GPR120) differentially regulates migration, invasion, proliferation and tumor growth of papillary renal cell carcinoma cells Biochemical pharmacology 2023-05-16 [PMID: 37201877]
Yu H, Yang W, Huang J et al. GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure Cell death & disease 2021-12-16 [PMID: 34911928] (FLOW, Mouse)	FLOW	Mouse
Ito T, Yamamoto Y, Yamagishi N, Kanai Y Stomach secretes estrogen in response to the blood triglyceride levels Communications biology 2021-12-07 [PMID: 34876651] (IHC-P, Rat)	IHC-P	Rat
Kamakura R, Raza GS, Prasannan A et al. Dipeptidyl peptidase 4 and GLP-1 interplay in STC-1 and GLUTag cell lines Peptides 2020-09-27 [PMID: 32998057] (WB, Mouse)	WB	Mouse
Bae IS, Kim SH Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes Int J Mol Sci 2019-12-05 [PMID: 31817347] (WB, Human)	WB	Human
Bodis K, Kahl S, Simon MC et al. Reduced expression of stearoyl-CoA desaturase-1, but not free fatty acid receptor 2 or 4 in subcutaneous adipose tissue of patients with newly diagnosed type 2 diabetes mellitus. Nutr Diabetes 2018-09-07 [PMID: 30190473] (Human)		Human
Liu D, Costanzo A, Evans MDM et al. Expression of the candidate fat taste receptors in human fungiform papillae and the association with fat taste function. Br. J. Nutr. 2018-07-01 [PMID: 29936924] (IF/IHC, Human)	IF/IHC	Human
Zhu S, Lin G, Song C et al. RA and O-3 PUFA co-treatment activates autophagy in cancer cells Oncotarget 2017-12-12 [PMID: 29312596] (WB, Human)	WB	Human
Kang S, Huang J, Lee BK et al. Omega-3 polyunsaturated fatty acids protect human hepatoma cells from developing steatosis through FFA4 (GPR120) Biochim. Biophys. Acta. 2017-11-07 [PMID: 29126901] (ICC/IF, Human)	ICC/IF	Human
Zhu S, Jiang X, Jiang S et al. GPR120 is not required for w-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells. Cell Biol. Int. 2017-10-05 [PMID: 28980737] (Human)		Human
Cheshmehkani A, Senatorov IS, Dhuguru J et al. Free-fatty acid receptor-4 (FFA4) modulates ROS generation and COX-2 expression via the C-terminal B-arrestin phosphosensor in Raw 264.7 macrophages Biochem. Pharmacol. 2017-09-21 [PMID: 28943238] (Mouse)		Mouse
Lee KP, Park SJ, Kang S et al. Omega-3 polyunsaturated fatty acids accelerate airway repair by activating FFA4 in club cells. Am. J. Physiol. Lung Cell Mol. Physiol. 2017-03-17 [PMID: 28314803] (WB, Mouse)	WB	Mouse
Houthuijzen JM, Oosterom I, Hudson BD et al. Fatty acid 16:4(n-3) stimulates a GPR120-induced signaling cascade in splenic macrophages to promote chemotherapy resistance. FASEB J. 2017-02-09 [PMID: 28183801]
Zhang D, So WY, Wang Y et al. Insulinotropic effects of GPR120 agonists are altered in obese non-diabetic and diabetic states. Clin. Sci. 2016-12-15 [PMID: 27980130] (Rat)		Rat
Baron M, Veres A, Wolock SL et al. A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure. Cell Syst 2016-10-26 [PMID: 27667365] (IF/IHC, Mouse)	IF/IHC	Mouse
Lee J, Moraes-Vieira Pm, Castoldi A et al. Branched fatty acid esters of hydroxy fatty acids (FAHFAs) protect against colitis by regulating the gut innate and adaptive immune systems. J. Biol. Chem. 2016-08-29 [PMID: 27573241] (WB)	WB
Anbazhagan AN, Priyamvada S, Gujral T et al. A Novel Anti-inflammatory Role of GPR120 in Intestinal Epithelial Cells. Am. J. Physiol., Cell Physiol. 2016-01-20 [PMID: 26791484] (WB, ICC/IF, Mouse, Human, Rat) Details: GPR120 antibody was used at 1:75 dilution in immunofluorescent / ICC-IF staining of control or agonist-treated (GW9508 or TUG-891, 6 hr) STC-1 cells grown on 6-well transwell inserts. The antibody was used for WB also on lysates from human, mouse or rat's intestinal mucosal tissues or from Caco-2 and STC-1 cell lines grown under several different experimental conditions.	WB, ICC/IF	Mouse, Human, Rat
Kim JM, Lee KP, Park SJ et al. Omega-3 fatty acids induce Ca(2+) mobilization responses in human colon epithelial cell lines endogenously expressing FFA4. Acta Pharmacol. Sin. 2015-05-25 [PMID: 26005911] (ICC/IF, Human) Details: FFAR4/GPR120 antibody was used at 1:500 dilution in ICC-IF immunostaining assay for the detection of endogenous free fatty acid receptor 4 (FFA4) protein in HCT116 and HT-29 human colon cancer cells 30 min after exposing the cells to 50uM of alpha linolenic acid/LA, eicosapentaenoic acid / ELA or docosahexaenoic acid /DHA (Figure 1C and 1D).	ICC/IF	Human
Chung H, Lee YS, Mayoral R et al. Omega-3 fatty acids reduce obesity-induced tumor progression independent of GPR120 in a mouse model of postmenopausal breast cancer. Oncogene. 2014-09-15 [PMID: 25220417] (WB, Mouse)	WB	Mouse
Liu Y, Chen LY, Sokolowska M et al. The fish oil ingredient, docosahexaenoic acid, activates cytosolic phospholipase A2 via GPR120 receptor to produce prostaglandin E2 and plays an anti-inflammatory role in macrophages. Immunology 2014-03-28 [PMID: 24673159] (WB, Mouse)	WB	Mouse
Jiang W, Zhu Z, McGinley JN et al. Identification of a molecular signature underlying inhibition of mammary carcinoma growth by dietary N-3 Fatty acids Cancer Res 2012-08-01 [PMID: 22651929] (WB, Rat)	WB	Rat
Show All 21 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	04/02/2015	View Summary Application Western Blot Sample Tested whole cell lysates from mouse bone marrow, spleen, bone marrow derived DCs/macrophages. Species Mouse Lot NBP1-00858

Array NBP1-00858

• FFAR4/GPR120 Antibodies
• FFAR4/GPR120 ELISA Kits
• FFAR4/GPR120 Proteins